ArticleNature chemical biology2017
Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis.
Article in Nature chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Metabolic engineering strategies of Corynebacterium glutamicum for sulfur-containing amino acid production: current progress and future directions.World journal of microbiology & biotechnology · 2026Review
- The Curious Case of CysE: Diversity and Distribution of Serine Acetyltransferases in Bacteria.Proteins · 2026Article
- Construction and regulation of microbial cell factories for enhancing the biosynthesis of O-acetyl-l-homoserine in Escherichia coli W3110.The Biochemical journal · 2026Article
- ASMC: investigating the amino acid diversity of enzyme active sites.Bioinformatics (Oxford, England) · 2025Article
- Article
- Engineering of methionine-auxotrophMetabolic engineering communications · 2024Article
- pH-dependent regulation of an acidophilicApplied and environmental microbiology · 2024Article
- Conversion of methionine biosynthesis in Escherichia coli from trans- to direct-sulfurylation enhances extracellular methionine levels.Microbial cell factories · 2023Article
- A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century.Microbial biotechnology · 2023Review
- Copiotrophy in a Marine-Biofilm-Derived Roseobacteraceae Bacterium Can Be Supported by Amino Acid Metabolism and Thiosulfate Oxidation.International journal of molecular sciences · 2023Article
- The coralScience advances · 2022Article
- Sulfide-quinone oxidoreductase is required for cysteine synthesis and indispensable to mitochondrial health.Redox biology · 2021Article
- Article
- One-carbon metabolism, folate, zinc and translation.Microbial biotechnology · 2020Article
- GapMind: Automated Annotation of Amino Acid Biosynthesis.mSystems · 2020Article
- The acid-base-nucleophile catalytic triad in ABH-fold enzymes is coordinated by a set of structural elements.PloS one · 2020Article
- Structural analysis of mycobacterial homoserine transacetylases central to methionine biosynthesis reveals druggable active site.Scientific reports · 2019Article
- Evidence for novel epigenetic marks within plants.AIMS genetics · 2019Article
- Revisiting the methionine salvage pathway and its paralogues.Microbial biotechnology · 2019Article
- No wisdom in the crowd: genome annotation in the era of big data - current status and future prospects.Microbial biotechnology · 2018Review
Corrections and comments
- Erratum issued
Authors and funding
21 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Experimental validation of enzyme function is crucial for genome interpretation, but it remains challenging because it cannot be scaled up to accommodate the constant accumulation of genome sequences. We tackled this issue for the MetA and MetX enzyme families, phylogenetically unrelated families of acyl-L-homoserine transferases involved in L-methionine biosynthesis. Members of these families are prone to incorrect annotation because MetX and MetA enzymes are assumed to always use acetyl-CoA and succinyl-CoA, respectively. We determined the enzymatic activities of 100 enzymes from diverse species, and interpreted the results by structural classification of active sites based on protein structure modeling. We predict that >60% of the 10,000 sequences from these families currently present in databases are incorrectly annotated, and suggest that acetyl-CoA was originally the sole substrate of these isofunctional enzymes, which evolved to use exclusively succinyl-CoA in the most recent bacteria. We also uncovered a divergent subgroup of MetX enzymes in fungi that participate only in L-cysteine biosynthesis as O-succinyl-L-serine transferases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.