ArticleCellular microbiology2017
The midgut microbiota plays an essential role in sand fly vector competence for Leishmania major.
Article in Cellular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
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Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- Can floral nectars reduce transmission of Leishmania?PLoS neglected tropical diseases · 2022Pooled it
- Baseline Characterization of the Gut Microbiota of Field and Colony Populations ofPathogens (Basel, Switzerland) · 2026Article
- Negative interactions between Toscana virus and Leishmania infantum limit coinfection in sand flies.Parasites & vectors · 2026Article
- Ecdysone signaling acts as a regulator of immunity and microbiota balance, shapingFrontiers in immunology · 2026Article
- Sand fly gut microbiota in Leishmania transmission: roles, interactions, and paratransgenic prospects.Archives of microbiology · 2025Review
- Characterization of Gut Bacteria in Natural Populations of Sand Flies (Microorganisms · 2025Article
- Microbiome diversity in mosquitoes and sand flies: implications for vector competence.Parasites & vectors · 2025Review
- Circulation aspects of phlebotomines (Diptera:Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Article
- Investigation of bacterial microbiota variability in two allopatric populations of Nyssomyia umbratilis, susceptible and nonsusceptible to Leishmania (Viannia) guyanensis infection in the Amazon region.Parasites & vectors · 2025Article
- Comparative Gut Proteome ofMicroorganisms · 2025Article
- Leishmania sand fly-transmission is disrupted by Delftia tsuruhatensis TC1 bacteria.Nature communications · 2025Article
- Detection of Leishmania metacyclogenesis within the sand fly vector employing a real-time PCR for sherp gene expression: A tool for Leishmania surveillance and transmission potential.PLoS neglected tropical diseases · 2025Article
- Article
- Recent advances in Phlebotomine sand fly research: a review based on studies presented at ISOPS XI.Parasite (Paris, France) · 2025Review
- Review
- Comparative analysis of the microbiota of sand fly vectors of Leishmania major and L. tropica in a mixed focus of cutaneous leishmaniasis in southeast Tunisia; ecotype shapes the bacterial community structure.PLoS neglected tropical diseases · 2024Article
- Effects of Leishmania major infection on the gut microbiome of resistant and susceptible mice.Applied microbiology and biotechnology · 2024Article
- High temperatures augment inhibition of parasites by a honey bee gut symbiont.Applied and environmental microbiology · 2023Article
- Microbiota in disease-transmitting vectors.Nature reviews. Microbiology · 2023Review
- Effects of host species on microbiota composition in Phlebotomus and Lutzomyia sand flies.Parasites & vectors · 2023Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 3 countries.
Funding
Abstract
For many arthropod vectors, the diverse bacteria and fungi that inhabit the gut can negatively impact pathogen colonization. Our attempts to exploit antibiotic treatment of colonized Phlebotomus duboscqi sand flies in order to improve their vector competency for Leishmania major resulted instead in flies that were refractory to the development of transmissible infections due to the inability of the parasite to survive and to colonize the anterior midgut with infective, metacyclic stage promastigotes. The parasite survival and development defect could be overcome by feeding the flies on different symbiont bacteria but not by feeding them on bacterial supernatants or replete medium. The inhibitory effect of the dysbiosis was moderated by lowering the concentration of sucrose (<30% w/v) used in the sugar feeds to maintain the colony. Exposure of promastigotes to 30% sucrose was lethal to the parasite in vitro. Confocal imaging revealed that the killing in vivo was confined to promastigotes that had migrated to the anterior plug region, corresponding to the highest concentrations of sucrose. The data suggest that sucrose utilization by the microbiota is essential to promote the appropriate osmotic conditions required for the survival of infective stage promastigotes in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.