ArticleBone reports2016
Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry.
Article in Bone reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Dry Deposition Strategies for MALDI-MS Imaging: Principles, Advances and Emerging Applications.Analytical science advances · 2026Review
- Tissue and extracellular matrix remodeling of the subchondral bone during osteoarthritis of knee joints as revealed by spatial mass spectrometry imaging.Bone research · 2026Article
- Bioenergetic programs of cancellous and cortical bone are distinct and differ with age and mechanical loading.Scientific reports · 2025Article
- Comprehensive High-Spatial-Resolution Imaging Metabolomics Workflow for Heterogeneous Tissues.Analytical chemistry · 2025Article
- In situ metabolomic analysis of osteonecrosis of the femoral head (ONFH) using MALDI MSI.Analytical and bioanalytical chemistry · 2024Article
- Mass Spectrometry Reveals Molecular Effects of Citrulline Supplementation during Bone Fracture Healing in a Rat Model.Journal of the American Society for Mass Spectrometry · 2024Article
- Sample Preparation Method for MALDI Mass Spectrometry Imaging of Fresh-Frozen Spines.Analytical chemistry · 2023Article
- High-ResolutionAnalytical chemistry · 2023Article
- Spatial Lipidomic Profiling of Mouse Joint Tissue Demonstrates the Essential Role of PHOSPHO1 in Growth Plate Homeostasis.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- Nutrient metabolism of the nucleus pulposus: A literature review.North American Spine Society journal · 2023Article
- Spatial metabolomics reveals upregulation of several pyrophosphate-producing pathways in cortical bone of Hyp mice.JCI insight · 2022Article
- Spatial quantitation of antibiotics in bone tissue compartments by laser-capture microdissection coupled with UHPLC-tandem mass spectrometry.Analytical and bioanalytical chemistry · 2022Article
- High Spatial Resolution MALDI Imaging Mass Spectrometry of Fresh-Frozen Bone.Analytical chemistry · 2022Article
- Mineralization of 3D Osteogenic Model Based on Gelatin-Dextran Hybrid Hydrogel Scaffold Bioengineered with Mesenchymal Stromal Cells: A Multiparametric Evaluation.Materials (Basel, Switzerland) · 2021Article
- Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications.Analytical and bioanalytical chemistry · 2021Article
- Modified application of Kawamoto's film method for super-resolution imaging of megakaryocytes in undecalcified bone marrow.Research and practice in thrombosis and haemostasis · 2020Article
- Application of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging for Food Analysis.Foods (Basel, Switzerland) · 2019Review
- Mass spectrometry imaging to detect lipid biomarkers and disease signatures in cancer.Cancer reports (Hoboken, N.J.) · 2019Review
- Advancements in composition and structural characterization of bone to inform mechanical outcomes and modelling.Current opinion in biomedical engineering · 2019Article
- How To Build a Bone: PHOSPHO1, Biomineralization, and Beyond.JBMR plus · 2019Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is an advanced method used globally to analyze the distribution of biomolecules on tissue cryosections without any probes. In bones, however, hydroxyapatite crystals make it difficult to determine the distribution of biomolecules using MALDI-IMS. Additionally, there is limited information regarding the use of this method to analyze bone tissues. To determine whether MALDI-IMS analysis of bone tissues can facilitate comprehensive mapping of biomolecules in mouse bone, we first dissected femurs and tibiae from 8-week-old male mice and characterized the quality of multiple fixation and decalcification methods for preparation of the samples. Cryosections were mounted on indium tin oxide-coated glass slides, dried, and then a matrix solution was sprayed on the tissue surface. Images were acquired using an iMScope at a mass-to-charge range of 100-1000. Hematoxylin-eosin, Alcian blue, Azan, and periodic acid-Schiff staining of adjacent sections was used to evaluate histological and histochemical features. Among the various fixation and decalcification conditions, sections from trichloroacetic acid-treated samples were most suitable to examine both histology and comprehensive MS images. However, histotypic MS signals were detected in all sections. In addition to the MS images, phosphocholine was identified as a candidate metabolite. These results indicate successful detection of biomolecules in bone using MALDI-IMS. Although analytical procedures and compositional adjustment regarding the performance of the device still require further development, IMS appears to be a powerful tool to determine the distribution of biomolecules in bone tissues.
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