ArticleFungal genetics and biology : FG & B2017
Fluorescent markers of various organelles in the wheat pathogen Zymoseptoria tritici.
Article in Fungal genetics and biology : FG & B, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Review
- Life on a leaf: the epiphyte to pathogen continuum and interplay in the phyllosphere.BMC biology · 2024Review
- Azoles activate type I and type II programmed cell death pathways in crop pathogenic fungi.Nature communications · 2024Article
- A bird's-eye view of the endoplasmic reticulum in filamentous fungi.Microbiology and molecular biology reviews : MMBR · 2024Review
- Use of chitin:DNA ratio to assess growth form in fungal cells.BMC biology · 2024Article
- Multi-site fungicides suppress banana Panama disease, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4.PLoS pathogens · 2022Article
- Zymoseptoria tritici white-collar complex integrates light, temperature and plant cues to initiate dimorphism and pathogenesis.Nature communications · 2022Article
- Conditional promoters to investigate gene function during wheat infection by Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2021Article
- Fungi, fungicide discovery and global food security.Fungal genetics and biology : FG & B · 2020Article
- Article
- The fungicide dodine primarily inhibits mitochondrial respiration in Ustilago maydis, but also affects plasma membrane integrity and endocytosis, which is not found in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2020Article
- The role of vegetative cell fusions in the development and asexual reproduction of the wheat fungal pathogen Zymoseptoria tritici.BMC biology · 2020Article
- Class V chitin synthase and β(1,3)-glucan synthase co-travel in the same vesicle in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2020Article
- CRISPR/Cas9-mediated endogenous gene tagging in Fusarium oxysporum.Fungal genetics and biology : FG & B · 2019Article
- ATP prevents Woronin bodies from sealing septal pores in unwounded cells of the fungus Zymoseptoria tritici.Cellular microbiology · 2017Article
- A role for random, humidity-dependent epiphytic growth prior to invasion of wheat by Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2017Article
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4 authors.
Funding
Abstract
Development of novel strategies to control fungal plant pathogens requires understanding of their cellular organisation and biology. Live cell imaging of fluorescent organelle markers has provided valuable insight into various aspects of their cell biology, including invasion strategies in plant pathogenic fungi. Here, we introduce a set of 17 vectors that encode fluorescent markers to visualize the plasma membrane, endoplasmic reticulum (ER), chromosomes, the actin cytoskeleton, peroxisomes and autophagosomes in the wheat pathogen Zymoseptoria tritici. We fused either enhanced green-fluorescent protein (eGFP) or a codon-optimised version of GFP (ZtGFP) to homologues of a plasma membrane-located Sso1-like syntaxin, an ER signalling and retention peptide, a histone H1 homologue, the LifeAct actin-binding peptide, a mitochondrial acetyl-CoA dehydrogenase, a peroxisomal import signal and a homologue of the ubiquitin-like autophagosomal protein Atg8. We expressed these markers in wildtype strain IPO323 and confirmed the specificity of these markers by counterstaining or physiological experiments. This new set of molecular tools will help understanding the cell biology of the wheat pathogen Z. tritici.
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