Evidence map›Paper›PMID 28578407›Full record

ArticleScientific reports2017

RNAi Screen and Proteomics Reveal NXF1 as a Novel Regulator of IRF5 Signaling.

Bishi Fu, Mengmeng Zhao, Lingyan Wang, Girish Patil, Jennifer A Smith, Ignacio J Juncadella, Ljiljana Zuvela-Jelaska, Martin E Dorf, Shitao Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Bishi FuDepartment of Microbiology & Immunobiology, Harvard Medical School, Boston, Massachusetts, 02115, USA.
Mengmeng ZhaoDepartment of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, 74078, USA.
Lingyan WangDepartment of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, 74078, USA.
Girish PatilDepartment of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, 74078, USA.
Jennifer A SmithDepartment of Microbiology & Immunobiology, Harvard Medical School, Boston, Massachusetts, 02115, USA.
Ignacio J JuncadellaDepartment of Immunology and Respiratory Diseases Research, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, Connecticut, 06877, USA.
Ljiljana Zuvela-JelaskaDepartment of Immunology and Respiratory Diseases Research, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, Connecticut, 06877, USA.
Martin E DorfDepartment of Microbiology & Immunobiology, Harvard Medical School, Boston, Massachusetts, 02115, USA. dorf@hms.harvard.edu.
Shitao LiDepartment of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, 74078, USA. shitao.li@okstate.edu.
Oklahoma State University · USBoehringer Ingelheim (United States) · USHarvard University · USMassachusetts Mental Health Center · US

Funding

ZFC3H1 Regulation of Host Defense and Influenza A Virus PathogenesisP20GM103648 · NIGMS · OKLAHOMA STATE UNIVERSITY STILLWATER · PI PATRAUCHAN, MARIANNA M. A. · 2013 to 2022
$22.3M
Role of TBK1 polyubiquitination in innate antiviral immunityR01AI089829 · NIAID · HARVARD MEDICAL SCHOOL · PI DORF, MARTIN E · 2011 to 2015
$2.6M
Interferon-induced IFITM recruitment of ZMPSTE24 blocks viral endocytic entryR01AI121288 · NIAID · HARVARD MEDICAL SCHOOL · PI DORF, MARTIN E · 2016 to 2019
$2.1M
Taxilin Alpha Regulates DNA-Mediated and Interferon-Dependent Innate ImmunityR15AI126360 · NIAID · OKLAHOMA STATE UNIVERSITY STILLWATER · PI LI, SHITAO · 2017 to 2017
$432k
NIAID NIH HHS R01 AI089829NIAID NIH HHS R01 AI121288NIAID NIH HHS R15 AI126360NIGMS NIH HHS P20 GM103648
6 · The paper itself

Abstract

Interferon regulatory factor 5 (IRF5) is a key transcription factor of innate immunity, which plays an important role in host restriction to viral infection and inflammation. Genome-wide association studies have implied the association of IRF5 with several autoimmune diseases, including systemic lupus erythematosus (SLE), Sjogren's syndrome, inflammatory bowel disease and multiple sclerosis. However, the regulation of IRF5-mediated immunity is not well understood. To uncover new regulators in IRF5 pathway, we used two "omics" approaches: affinity purification coupled with mass spectrometry and a high throughput RNAi screen. Proteomics identified 16 new IRF5 interactors while RNAi-mediated knockdown found 43 regulators of the TLR7-dependent IRF5 signaling pathway. NXF1 was identified in both screens. Stimulation with TLR7 ligand enhances formation of IRF5-NXF1 protein complexes. Gain or loss-of-function experiments revealed NXF1 selectively regulates TLR7-driven IRF5 transcriptional activity, suggesting a new role for NXF1 in the IRF5 signaling pathway.

Indexed as

ProteomicsRNA InterferenceSignal TransductionGenes, ReporterHumansImmunity, InnateInterferon Regulatory FactorsInterferonsNucleocytoplasmic Transport ProteinsProteasome Endopeptidase ComplexProtein BindingProtein Interaction MappingProtein Interaction MapsProtein IsoformsReproducibility of ResultsRNA-Binding ProteinsInterferon Regulatory FactorsInterferonsIRF5 protein, humanNucleocytoplasmic Transport ProteinsNXF1 protein, humanProteasome Endopeptidase ComplexProtein IsoformsRNA-Binding Proteins

Identifiers

PMID28578407
PMCPMC5457443
OpenAlexW2618221874

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.