ArticleScientific reports2017
The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Recombinant ubbR promoter enables highly efficient tamoxifen-inducible Cre recombination in embryonic and adult zebrafish.Genetics · 2026Article
- Macrophages and cardiac lesion in zebrafish: what can single-cell RNA sequencing reveal?Frontiers in cardiovascular medicine · 2025Review
- Harnessing the regenerative potential of interleukin11 to enhance heart repair.Nature communications · 2024Article
- The innate immune regulator MyD88 dampens fibrosis during zebrafish heart regeneration.Nature cardiovascular research · 2024Article
- Harnessing the regenerative potential ofbioRxiv : the preprint server for biology · 2024Article
- A Multimodal Omics Framework to Empower Target Discovery for Cardiovascular Regeneration.Cardiovascular drugs and therapy · 2024Review
- Endothelial Brg1 fine-tunes Notch signaling during zebrafish heart regeneration.NPJ Regenerative medicine · 2023Article
- The regenerative response of cardiac interstitial cells.Journal of molecular cell biology · 2023Article
- Ccn2a-FGFR1-SHH signaling is necessary for intervertebral disc homeostasis and regeneration in adult zebrafish.Development (Cambridge, England) · 2023Article
- Zebrafish heart regeneration after coronary dysfunction-induced cardiac damage.Developmental biology · 2022Article
- Zebrafish as a Model to Study Vascular Elastic Fibers and Associated Pathologies.International journal of molecular sciences · 2022Review
- Modulation of VEGFA Signaling During Heart Regeneration in Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Article
- Multiplexed bio-imaging using cadmium telluride quantum dots synthesized by mathematically derived process parameters in a continuous flow active microreactor.Materials today. Bio · 2021Article
- The Zebrafish Cardiac Endothelial Cell-Roles in Development and Regeneration.Journal of cardiovascular development and disease · 2021Review
- Zebrafish as a New Tool in Heart Preservation Research.Journal of cardiovascular development and disease · 2021Review
- Ccn2a is an injury-induced matricellular factor that promotes cardiac regeneration in zebrafish.Development (Cambridge, England) · 2021Article
- Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation.Developmental cell · 2019Article
- Immune responses in cardiac repair and regeneration: a comparative point of view.Cellular and molecular life sciences : CMLS · 2019Review
- Coronary Vasculature in Cardiac Development and Regeneration.Journal of cardiovascular development and disease · 2018Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite our increasing understanding of zebrafish heart development and regeneration, there is limited information about the distribution of endothelial cells (ECs) in the adult zebrafish heart. Here, we investigate and compare the distribution of cardiac ECs (cECs) in adult mouse and zebrafish ventricles. Surprisingly, we find that (i) active coronary vessel growth is present in adult zebrafish, (ii) ~37 and ~39% of cells in the zebrafish heart are ECs and cardiomyocytes, respectively, a composition similar to that seen in mouse. However, we find that in zebrafish, ~36% of the ventricular tissue is covered with ECs, i.e., a substantially larger proportion than in mouse. Capitalising on the high abundance of cECs in zebrafish, we established a protocol to isolate them with high purity using fluorescent transgenic lines. Our approach eliminates side-effects due to antibody utilisation. Moreover, the isolated cECs maintained a high proliferation index even after three passages and were amenable to pharmacological treatments to study cEC migration in vitro. Such primary cultures will be a useful tool for supplementary in vitro studies on the accumulating zebrafish mutant lines as well as the screening of small molecule libraries on cardiac specific endothelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.