Evidence map›Paper›PMID 28577989›Full record

ArticleNeurochemistry international2017

Hemicholinium-3 sensitive choline transport in human T lymphocytes: Evidence for use as a proxy for brain choline transporter (CHT) capacity.

Ajeesh Koshy Cherian, Vinay Parikh, Qi Wu, Yang Mao-Draayer, Qin Wang, Randy D Blakely, Martin Sarter

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurochemistry international, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
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  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Ajeesh Koshy CherianDept. of Psychology & Neuroscience Program, University of Michigan, Ann Arbor, MI, USA.
Vinay ParikhDepartment of Psychology Neuroscience Program, Temple University, Philadelphia, PA, USA.
Qi WuDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Yang Mao-DraayerDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Qin WangDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Randy D BlakelyBrain Institute and Department of Biomedical Sciences and Brain Institute, Charles E. Schmidt College of Medicine, Florida Atlantic University, Jupiter, FL, USA.
Martin SarterDept. of Psychology & Neuroscience Program, University of Michigan, Ann Arbor, MI, USA. Electronic address: msarter@umich.edu.
University of Michigan · USFlorida Atlantic University · USTemple University · US

Funding

Public Outreach and Education CoreP50NS091856 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ALBIN, ROGER L · 2014 to 2019
$10.1M
Molecular Analysis of Presynaptic Choline TransportersR37MH073159 · NIMH · VANDERBILT UNIVERSITY · PI BLAKELY, RANDY D. · 2004 to 2013
$3.6M
Choline transporter capacity limits motivated behavior on mice, rats, and humansR01MH086530 · NIMH · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SARTER, MARTIN F · 2010 to 2014
$1.9M
CD24 Targeting for Multiple Sclerosis: a First-In-Human Phase I StudyR01NS080821 · NINDS · ONCOIMMUNE, INC. · PI LIU, YANG · 2013 to 2014
$1.1M
University of Michigan Clinical Autoimmunity Center of ExcellenceUM1AI144298 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FOX, DAVID ALAN, KHANNA, DINESH · 2019 to 2023
$546k
University of Michigan Autoimmunity Center of Excellence, Clinical Research ProgrUM1AI110557 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FOX, DAVID ALAN · 2014 to 2018
$543k
NIAID NIH HHS UM1 AI110557NIMH NIH HHS R01 MH086530NIMH NIH HHS R37 MH073159NINDS NIH HHS P50 NS091856NINDS NIH HHS R01 NS080821
6 · The paper itself

Abstract

The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. To advance research on the impact of CHT capacity in humans, we established the presence of the neuronal CHT protein in human T lymphocytes. Next, we demonstrated CHT-mediated choline transport in human T cells. To address the validity of T cell-based choline uptake as a proxy for brain CHT capacity, we isolated T cells from the spleen, and synaptosomes from cortex and striatum, of wild type and CHT-overexpressing mice (CHT-OXP). Choline uptake capacity in T cells from CHT-OXP mice was two-fold higher than in wild type mice, mirroring the impact of CHT over-expression on synaptosomal CHT-mediated choline uptake. Monitoring T lymphocyte CHT protein and activity may be useful for estimating human CNS cholinergic capacity and for testing hypotheses concerning the contribution of CHT and, more generally, ACh signaling in cognition, neuroinflammation and disease.

Indexed as

AnimalsBiological TransportBrainCholineDose-Response Relationship, DrugFemaleHemicholinium 3HumansLeukocytes, MononuclearMaleMembrane Transport ProteinsMiceMice, Inbred C57BLMice, TransgenicT-LymphocytesCholinecholine transporterHemicholinium 3Membrane Transport ProteinsAcetylcholineCholine transporterGenetic CHT variantsSynaptosomesT lymphocytes

Identifiers

PMID28577989
PMCPMC5524217
OpenAlexW2621208522

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.