ArticleInternational journal for parasitology2017
Heritable genetic transformation of Strongyloides stercoralis by microinjection of plasmid DNA constructs into the male germline.
Article in International journal for parasitology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Transgenesis and targeted mutagenesis in the human-parasitic nematodeFrontiers in parasitology · 2026Review
- Pelodera: cosmopolitan phoretic saprotrophs and neglected models for origins of nematode parasitism.Parasites & vectors · 2025Review
- CRISPR-based functional genomics for schistosomes and related flatworms.Trends in parasitology · 2024Review
- Introduction toJournal of nematology · 2024Article
- Invade or die: behaviours and biochemical mechanisms that drive skin penetration inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Using newly optimized genetic tools to probe Strongyloides sensory behaviors.Molecular and biochemical parasitology · 2022Review
- Localization and RNA Interference-Driven Inhibition of a Brugia malayi-Encoded Interleukin-5 Receptor Binding Protein.Infection and immunity · 2022Article
- Generating Transgenics and Knockouts in Strongyloides Species by Microinjection.Journal of visualized experiments : JoVE · 2021Article
- Transcriptional profiles in Strongyloides stercoralis males reveal deviations from the Caenorhabditis sex determination model.Scientific reports · 2021Article
- Identification of a nuclear receptor/coactivator developmental signaling pathway in the nematode parasiteProceedings of the National Academy of Sciences of the United States of America · 2021Article
- The role of carbon dioxide in nematode behaviour and physiology.Parasitology · 2020Review
- Recent advances in functional genomics for parasitic nematodes of mammals.The Journal of experimental biology · 2020Review
- Advances in the Molecular and Cellular Biology ofCurrent tropical medicine reports · 2019Article
- Omics Driven Understanding of the Intestines of Parasitic Nematodes.Frontiers in genetics · 2019Review
- CRISPR/Cas9 Mutagenesis and Expression of Dominant Mutant Transgenes as Functional Genomic Approaches in Parasitic Nematodes.Frontiers in genetics · 2019Review
- Targeted mutagenesis in a human-parasitic nematode.PLoS pathogens · 2017Article
- Different but overlapping populations of Strongyloides stercoralis in dogs and humans-Dogs as a possible source for zoonotic strongyloidiasis.PLoS neglected tropical diseases · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Heretofore, transgenesis in the parasitic nematode genus Strongyloides has relied on microinjecting transgene constructs into gonadal syncytia of free-living females. We now report transgenesis in Strongyloides stercoralis by microinjecting constructs into the syncytial testes of free-living males. Crosses of individual males microinjected with a construct encoding GFP with cohorts of 12 non-injected females produced a mean of 7.28±2.09 transgenic progeny. Progeny of males and females microinjected with distinct reporter constructs comprised 2.6%±0.7% of individuals expressing both paternal and maternal transgenes. Implications of this finding for deployment of CRISPR/Cas9 mutagenesis in Strongyloides spp. are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.