Evidence map›Paper›PMID 28577236›Full record

ArticlePlant cell reports2017

Glutathione S-transferase related detoxification processes are correlated with receptor-mediated vacuolar sorting mechanisms.

Fabrizio Barozzi, Gian-Pietro Di Sansebastiano, Erika Sabella, Alessio Aprile, Gabriella Piro, Luigi De Bellis, Eliana Nutricati

Abstract read
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In one paragraph

Article in Plant cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Endomembrane Reorganization Induced by Heavy Metals.Plants (Basel, Switzerland) · 2020
    Review
  5. Article
  6. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Fabrizio BarozziDISTEBA, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Prov. le Lecce-Monteroni, 73100, Lecce, Italy.
Gian-Pietro Di SansebastianoDISTEBA, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Prov. le Lecce-Monteroni, 73100, Lecce, Italy.
Erika SabellaDISTEBA, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Prov. le Lecce-Monteroni, 73100, Lecce, Italy.
Alessio AprileDISTEBA, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Prov. le Lecce-Monteroni, 73100, Lecce, Italy.
Gabriella PiroDISTEBA, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Prov. le Lecce-Monteroni, 73100, Lecce, Italy.
Luigi De BellisDISTEBA, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Prov. le Lecce-Monteroni, 73100, Lecce, Italy.
Eliana NutricatiDISTEBA, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Prov. le Lecce-Monteroni, 73100, Lecce, Italy. eliana.nutricati@unisalento.it.ORCID http://orcid.org/0000-0002-1197-5693
University of Salento · IT

Funding

Regione Puglia Project Reti di Laboratorio Pubblici di ricerca "SELGE"
6 · The paper itself

Abstract

key messageTriticum durum Glutathione S-transferase Z1 is specifically responsive to glyphosate. Its expression influences the receptor-mediated vacuolar sorting mechanisms involved in tolerance mechanisms. A zeta subfamily glutathione S-transferase gene from Triticum durum (cv Cappelli) (TdGSTZ1) was characterized as part of a complex detoxification mechanism. The effect of different abiotic stresses on TdGSTZ1 revealed that the gene is unexpectedly responsive to glyphosate (GLY) herbicide despite it should not be part of tolerance mechanisms. Its role in the non-target-site mechanism of GLY resistance was then investigated. To analyze the GLY and the TdGSTZ1 overexpression effects on vacuolar sorting mechanisms, we performed transient transformation experiments in Nicotiana tabacum protoplasts using two vacuolar markers, AleuGFPgl133 and GFPgl133Chi, labeling the Sar1 dependent or independent sorting, respectively. We observed that the adaptive reaction of tobacco protoplasts vacuolar system to the treatment with GLY could be partially mimicked by the overexpression of TdGSTZ1 gene. To confirm the influence of GLY on the two vacuolar markers accumulation and the potential involvement of the secretion pathway activity in detoxification events, Arabidopsis thaliana transgenic plants overexpressing the non-glycosylated versions of the two markers were analyzed. The results suggested that GLY treatment specifically altered different vacuolar sorting characteristics, suggesting an involvement of the receptor-mediated AleuGFP sorting mechanism in GLY resistance. Finally, the expression analysis of selected genes confirmed that the non-target-site GLY resistance mechanisms are related to vacuolar sorting.

Indexed as

Inactivation, MetabolicGene Expression Regulation, PlantGlutathione TransferaseGlycineGlyphosateNicotianaPlant ProteinsPlants, Genetically ModifiedProtein TransportProtoplastsReceptors, Cell SurfaceTriticumVacuolesGlutathione TransferaseGlycineGlyphosatePlant ProteinsReceptors, Cell SurfaceAleuGFPgl133GFPgl133ChiGlyphosateTdGSTZ1Vacuolar markers

Identifiers

PMID28577236
OpenAlexW2621312510

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.