Evidence map›Paper›PMID 28576885›Full record

ArticleBone & joint research2017

Osteoporosis and ageing affects the migration of stem cells and this is ameliorated by transfection with CXCR4.

A Sanghani-Kerai, M Coathup, S Samazideh, P Kalia, L Di Silvio, B Idowu, G Blunn

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bone & joint research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
4.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 43 citations in OpenAlex.

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  13. VEGF with AMD3100 endogenously mobilizes mesenchymal stem cells and improves fracture healing.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

A Sanghani-KeraiThe John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, Division of Surgery and Interventional Sciences, University College London, Stanmore, Middlesex HA7 4LP, UK a.sanghani.12@ucl.ac.uk.
M CoathupThe John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, Division of Surgery and Interventional Sciences, University College London, Stanmore, Middlesex HA7 4LP, UK.
S SamazidehThe John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, Division of Surgery and Interventional Sciences, University College London, Stanmore, Middlesex HA7 4LP, UK.
P KaliaDepartment of Tissue Engineering and Biophotonics, Guy's Hospital, London SE1 9RT, UK.
L Di SilvioDepartment of Tissue Engineering and Biophotonics, Guy's Hospital, London SE1 9RT, UK.
B IdowuDepartment of Tissue Engineering and Biophotonics, Guy's Hospital, London SE1 9RT, UK.
G BlunnThe John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, Division of Surgery and Interventional Sciences, University College London, Stanmore, Middlesex HA7 4LP, UK.
Guy's Hospital · GBUniversity College London · GBStanmore College · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesCellular movement and relocalisation are important for many physiologic properties. Local mesenchymal stem cells (MSCs) from injured tissues and circulating MSCs aid in fracture healing. Cytokines and chemokines such as Stromal cell-derived factor 1(SDF-1) and its receptor chemokine receptor type 4 (CXCR4) play important roles in maintaining mobilisation, trafficking and homing of stem cells from bone marrow to the site of injury. We investigated the differences in migration of MSCs from the femurs of young, adult and ovariectomised (OVX) rats and the effect of CXCR4 over-expression on their migration.

methodsMSCs from young, adult and OVX rats were put in a Boyden chamber to establish their migration towards SDF-1. This was compared with MSCs transfected with CXCR4, as well as MSCs differentiated to osteoblasts.

resultsMSCs from OVX rats migrate significantly (p < 0.05) less towards SDF-1 (9%, sd 5%) compared with MSCs from adult (15%, sd 3%) and young rats (25%, sd 4%). Cells transfected with CXCR4 migrated significantly more towards SDF-1 compared with non-transfected cells, irrespective of whether these cells were from OVX (26.5%, sd 4%), young (47%, sd 17%) or adult (21%, sd 4%) rats. Transfected MSCs differentiated to osteoblasts express CXCR4 but do not migrate towards SDF-1.

conclusionsMSC migration is impaired by age and osteoporosis in rats, and this may be associated with a significant reduction in bone formation in osteoporotic patients. The migration of stem cells can be ameliorated by upregulating CXCR4 levels which could possibly enhance fracture healing in osteoporotic patients.

Indexed as

BoneMesenchymalOsteoporosis

Identifiers

PMID28576885
PMCPMC5492335
OpenAlexW2621038067

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.