Evidence map›Paper›PMID 28575156›Full record

ArticleBiology of reproduction2017

Consequences of endogenous and exogenous WNT signaling for development of the preimplantation bovine embryo.

Paula Tribulo, Beatriz Caetano da Silva Leão, Khoboso C Lehloenya, Gisele Zoccal Mingoti, Peter J Hansen

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of reproduction, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 54 citations in OpenAlex.

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  17. Pluripotent Core in Bovine Embryos: A Review.Animals : an open access journal from MDPI · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 3 countries.

Paula TribuloDepartment of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, Florida, USA.
Beatriz Caetano da Silva LeãoSchool of Veterinary Medicine, Laboratory of Reproductive Physiology, UNESP-Universidade Estadual Paulista, Araçatuba, São Paulo, Brazil and Post-Graduation Program in Veterinary Medicine, School of Agrarian and Veterinarian Sciences, Department of Animal Reproduction, UNESP-Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
Khoboso C LehloenyaDepartment of Animal and Wildlife Sciences, University of Pretoria, Pretoria, South Africa.
Gisele Zoccal MingotiSchool of Veterinary Medicine, Laboratory of Reproductive Physiology, UNESP-Universidade Estadual Paulista, Araçatuba, São Paulo, Brazil and Post-Graduation Program in Veterinary Medicine, School of Agrarian and Veterinarian Sciences, Department of Animal Reproduction, UNESP-Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
Peter J HansenDepartment of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, Florida, USA.
Universidade Estadual Paulista (Unesp) · BRUniversity of Florida · USUniversity of Pretoria · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The specific role of WNT signaling during preimplantation development remains unclear. Here, we evaluated consequences of activation and inhibition of β-catenin (CTNNB1)-dependent and -independent WNT signaling in the bovine preimplantation embryo. Activation of CTNNB1-mediated WNT signaling by the agonist 2-amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidine (AMBMP) and a glycogen synthase kinase 3 inhibitor reduced development to the blastocyst stage. Moreover, the antagonist of WNT signaling, dickkopf-related protein 1 (DKK1), alleviated the negative effect of AMBMP on development via reduction of CTNNB1. Based on labeling for phospho c-Jun N-terminal kinase, there was no evidence that DKK1 activated the planar cell polarity (PCP) pathway. Inhibition of secretion of endogenous WNTs did not affect development but increased number of cells in the inner cell mass (ICM). In contrast, DKK1 did not affect number of ICM or trophectoderm (TE) cells, suggesting that embryo-derived WNTs regulate ICM proliferation through a mechanism independent of CTNNB1. In addition, DKK1 did not affect the number of cells positive for the transcription factor yes-associated protein 1 (YAP1) involved in TE formation. In fact, DKK1 decreased YAP1. In contrast, exposure of embryos to WNT family member 7A (WNT7A) improved blastocyst development, inhibited the PCP pathway, and did not affect amounts of CTNNB1. Results indicate that embryo-derived WNTs are dispensable for blastocyst formation but participate in regulation of ICM proliferation, likely through a mechanism independent of CTNNB1. The response to AMBMP and WNT7A leads to the hypothesis that maternally derived WNTs can play a positive or negative role in regulation of preimplantation development.

Indexed as

Embryonic DevelopmentWnt Signaling PathwayAnimalsBenzodioxolesbeta CateninBlastocystCattleEmbryo Culture TechniquesGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicGlycogen Synthase Kinase 3Intercellular Signaling Peptides and ProteinsJNK Mitogen-Activated Protein KinasesPyridinesPyrimidines2-amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidineBenzodioxolesbeta CateninChir 99021Glycogen Synthase Kinase 3Intercellular Signaling Peptides and ProteinsJNK Mitogen-Activated Protein KinasesPyridinesPyrimidinesDKK1embryo developmentpreimplantation developmentWNTWNT7A

Identifiers

PMID28575156
PMCPMC5803770
OpenAlexW2618542783

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.