ArticlePloS one2017
A small molecule screen identifies in vivo modulators of peripheral nerve regeneration in zebrafish.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Modeling traumatic brain and neural injuries: insights from zebrafish.Frontiers in molecular neuroscience · 2025Review
- A screen of pharmacologically active compounds to identify modulators of the Adgrg6/Gpr126 signalling pathway in zebrafish embryos.Basic & clinical pharmacology & toxicology · 2023Article
- Prolonged Dexamethasone Exposure Enhances Zebrafish Lateral-Line Regeneration But Disrupts Mitochondrial Homeostasis and Hair Cell Function.Journal of the Association for Research in Otolaryngology : JARO · 2022Article
- Therapeutic Potential of CKD-504, a Novel Selective Histone Deacetylase 6 Inhibitor, in a Zebrafish Model of Neuromuscular Junction Disorders.Molecules and cells · 2022Article
- Anatomy and development of the pectoral fin vascular network in the zebrafish.Development (Cambridge, England) · 2022Article
- Schwann cell interactions during the development of the peripheral nervous system.Developmental neurobiology · 2021Review
- Current Advances in Comprehending Dynamics of Regenerating Axons and Axon-Glia Interactions after Peripheral Nerve Injury in Zebrafish.International journal of molecular sciences · 2021Review
- Insights Into Central Nervous System Glial Cell Formation and Function From Zebrafish.Frontiers in cell and developmental biology · 2021Review
- RNA-seq analysis and compound screening highlight multiple signalling pathways regulating secondary cell death after acute CNS injuryBiology open · 2020Article
- Systemic loss of Sarm1 protects Schwann cells from chemotoxicity by delaying axon degeneration.Communications biology · 2020Article
- Imaging in the repair of peripheral nerve injury.Nanomedicine (London, England) · 2019Article
- Let's get small (and smaller): Combining zebrafish and nanomedicine to advance neuroregenerative therapeutics.Advanced drug delivery reviews · 2019Review
- Developing zebrafish disease models for in vivo small molecule screens.Current opinion in chemical biology · 2019Review
- The ubiquitin ligase PHR promotes directional regrowth of spinal zebrafish axons.Communications biology · 2019Article
- HSP90 is a chaperone for DLK and is required for axon injury signaling.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Adult vertebrates have retained the ability to regenerate peripheral nerves after injury, although regeneration is frequently incomplete, often leading to functional impairments. Small molecule screens using whole organisms have high potential to identify biologically relevant targets, yet currently available assays for in vivo peripheral nerve regeneration are either very laborious and/or require complex technology. Here we take advantage of the optical transparency of larval zebrafish to develop a simple and fast pectoral fin removal assay that measures peripheral nerve regeneration in vivo. Twenty-four hours after fin amputation we observe robust and stereotyped nerve regrowth at the fin base. Similar to laser mediated nerve transection, nerve regrowth after fin amputation requires Schwann cells and FGF signaling, confirming that the fin amputation assay identifies pathways relevant for peripheral nerve regeneration. From a library of small molecules with known targets, we identified 21 compounds that impair peripheral nerve regeneration. Several of these compounds target known regulators of nerve regeneration, further validating the fin removal assay. Twelve of the identified compounds affect targets not previously known to control peripheral nerve regeneration. Using a laser-mediated nerve transection assay we tested ten of those compounds and confirmed six of these compounds to impair peripheral nerve regeneration: an EGFR inhibitor, a glucocorticoid, prostaglandin D2, a retinoic acid agonist, an inhibitor of calcium channels and a topoisomerase I inhibitor. Thus, we established a technically simple assay to rapidly identify valuable entry points into pathways critical for vertebrate peripheral nerve regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.