Article3 Biotech2017
High-fidelity PCR enzyme with DNA-binding domain facilitates de novo gene synthesis.
Article in 3 Biotech, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- A Restriction-Free Cloning Approach for Molecular Engineering of Plasmids.Methods in molecular biology (Clifton, N.J.) · 2027Article
- A Simple Method to Produce Recombinant Mammalian Serum Albumins inInternational journal of molecular sciences · 2026Article
- Development and evaluation of an assay for the detection of tick-borne encephalitis virus RNA via real-time PCR with reverse transcription.Parasites & vectors · 2026Article
- Article
- Development and evaluation of a real-time RT-PCR assay for Mammarenavirus guanaritoense virus detection.BMC infectious diseases · 2026Article
- Isothermal real-time RT-RPA for Machupo virus detection: Field-adaptable sensitivity comparable with laboratory PCR.PloS one · 2026Article
- CRISPR-Cas12a-Based Isothermal Detection ofInternational journal of molecular sciences · 2025Article
- Development and Evaluation of a New Measles Detection Assay Using Real-Time RT-PCR.International journal of molecular sciences · 2025Article
- Effects of combined cytotoxic T-lymphocyte antigen 4 and programed death 1 ligand-receptor blockade on interferon-gamma production in bovine leukemia virus-infected cattle.Veterinary world · 2024Article
- Development and Validation of a Protein Array for Detection of Antibodies against the Tick-Borne Pathogen Borrelia miyamotoi.Microbiology spectrum · 2022Article
- Recombinant Expression and Purification of Extracellular Domain of the Programmed Cell Death Protein Receptor.Reports of biochemistry & molecular biology · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nowadays enzymatic synthesis of genes is the most powerful tool for fast resolution of the various tasks in the field of basic and applied biological research. PCR-based gene assembly from overlapping oligonucleotides has become a widely used strategy. However, all the methods described in the literature are not perfect and need an extra processing step. In this study we are verifying Phusion high-fidelity polymerase as a tool to reduce nucleotide mismatches in de novo gene synthesis, thus facilitating subsequent cloning. To test the efficiency of the polymerase, we selected Fel d 4 gene, which is a 581 bp DNA sequence encoding the lipocalin allergen protein, one of the major cat allergens. The approach described here, therefore, would be useful in DNA sequences creation.
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Registered trials
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