Evidence map›Paper›PMID 28573341›Full record

ArticleCurrent microbiology2017

Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Using Simultaneous Detection of mecA, nuc, and femB by Loop-Mediated Isothermal Amplification (LAMP).

Changguo Chen, Qiangyuan Zhao, Jianwei Guo, Yanjun Li, Qiuyuan Chen

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 1 pooled it
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.

  1. Diagnostic accuracy of LAMP assay for HBV infection.Journal of clinical laboratory analysis · 2020
    Pooled it
  2. Review
  3. Mpox disease, diagnosis, and point of care platforms.Bioengineering & translational medicine · 2025
    Review
  4. Article
  5. Degree of Contamination of Gutta-Percha Points byInternational journal of molecular sciences · 2024
    Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Loop-mediated isothermal amplification (LAMP): a sensitive molecular tool for detection of Staphylococcus aureus in meat and dairy product.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022
    Article
  11. Article
  12. Article
  13. Article
  14. Comparison between Whatman FTA Elute Cards and Conventional Swab for the Detection of Pathogenic Enteric Bacteria Using an RT-qPCR Assay.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2021
    Article
  15. Article
  16. Detection ofSaudi journal of biological sciences · 2020
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Changguo ChenDepartment of Clinical Laboratory, The Navy General Hospital, No. 6 Fucheng Road, Beijing, 100037, People's Republic of China. 1234_chen@sina.com.
Qiangyuan ZhaoDepartment of Clinical Laboratory, The Navy General Hospital, No. 6 Fucheng Road, Beijing, 100037, People's Republic of China.
Jianwei GuoDepartment of Clinical Laboratory, The Navy General Hospital, No. 6 Fucheng Road, Beijing, 100037, People's Republic of China.
Yanjun LiDepartment of Clinical Laboratory, The Navy General Hospital, No. 6 Fucheng Road, Beijing, 100037, People's Republic of China.
Qiuyuan ChenDepartment of Clinical Laboratory, The Navy General Hospital, No. 6 Fucheng Road, Beijing, 100037, People's Republic of China.
PLA Navy General Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to develop a rapid detection assay to identify methicillin-resistant Staphylococcus aureus by simultaneous testing for the mecA, nuc, and femB genes using the loop-mediated isothermal amplification (LAMP) method. LAMP primers were designed using online bio-software ( http://primerexplorer.jp/e/ ), and amplification reactions were performed in an isothermal temperature bath. The products were then examined using 2% agarose gel electrophoresis. MecA, nuc, and femB were confirmed by triplex TaqMan real-time PCR. For better naked-eye inspection of the reaction result, hydroxy naphthol blue (HNB) was added to the amplification system. Within 60 min, LAMP successfully amplified the genes of interest under isothermal conditions at 63 °C. The results of 2% gel electrophoresis indicated that when the Mg

Indexed as

Bacterial ProteinsBacteriological TechniquesElectrophoresis, Agar GelMethicillin-Resistant Staphylococcus aureusMicrococcal NucleaseMolecular Diagnostic TechniquesNaphthalenesulfonatesNucleic Acid Amplification TechniquesPenicillin-Binding ProteinsStaining and LabelingStaphylococcal InfectionsBacterial ProteinsFemB protein, methicillin-resistant Staphylococcus aureusmecA protein, Staphylococcus aureusMicrococcal NucleaseNaphthalenesulfonatesnuc protein, staphylococcusPenicillin-Binding Proteinstrisodium 3-hydroxy-4-((2Z)-2-(2-oxo-4-sulfonatonaphthalen-1-ylidene)hydrazinyl)naphthalene-2,7-disulfonate

Identifiers

PMID28573341
OpenAlexW2620748783

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.