ArticleScientific reports2017
Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Structural and biochemical comparison of the FLVCR and CTL membrane protein families in eukaryotes.Life science alliance · 2026Article
- Molecular Dynamics Simulations of the Spike Protein Receptor Binding Domain Adsorption to Material Surfaces.The journal of physical chemistry. B · 2025Article
- Structural determination of small proteins by cryo-EM using a coiled coil module strategy.Scientific reports · 2025Article
- A fiducial-assisted strategy compatible with resolving small MFS transporter structures in multiple conformations using cryo-EM.Nature communications · 2025Article
- A method for structure determination of GPCRs in various states.Nature chemical biology · 2024Article
- The New Functional Hybrid Chaperone Protein ADGroEL-SacSm.Molecules (Basel, Switzerland) · 2023Article
- Exploring rigid-backbone protein docking in biologics discovery: a test using the DARPin scaffold.Frontiers in molecular biosciences · 2023Article
- Functionalisation of Inorganic Material Surfaces with Staphylococcus Protein A: A Molecular Dynamics Study.International journal of molecular sciences · 2022Article
- Successive remodeling of IgG glycans using a solid-phase enzymatic platform.Communications biology · 2022Article
- Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies).Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Design of multi-scale protein complexes by hierarchical building block fusion.Nature communications · 2021Article
- Allosteric cooperation in a de novo-designed two-domain protein.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Novel structure of the N-terminal helical domain of BibA, a group B streptococcus immunogenic bacterial adhesin.Acta crystallographica. Section D, Structural biology · 2020Article
- Modular repeat protein sculpting using rigid helical junctions.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Development of imaging scaffolds for cryo-electron microscopy.Current opinion in structural biology · 2020Review
- Elfin UI: A Graphical Interface for Protein Design With Modular Building Blocks.Frontiers in bioengineering and biotechnology · 2020Article
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7 authors.
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Abstract
Generating artificial protein assemblies with complex shapes requires a method for connecting protein components with stable and predictable structures. Currently available methods for creating rigid protein assemblies rely on either complicated calculations or extensive trial and error. We describe a simple and efficient method for connecting two proteins via a fused alpha helix that is formed by joining two preexisting helices into a single extended helix. Because the end-to-end ligation of helices does not guarantee the formation of a continuous helix, we superimposed 1-2 turns of pairs of connecting helices by using a molecular graphics program. Then, we chose amino acids from the two natural sequences that would stabilize the connecting helix. This "shared helix method" is highly efficient. All the designed proteins that could be produced in Escherichia coli were readily crystallized and had the expected fusion structures. To prove the usefulness of this method, we produced two novel repeat proteins by assembling several copies of natural or artificial proteins with alpha helices at both termini. Their crystal structures demonstrated the successful assembly of the repeating units with the intended curved shapes. We propose that this method could dramatically expand the available repertoire of natural repeat proteins.
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