ArticleScientific reports2017
Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Surra-affected dromedary camels show reduced numbers of blood B-cells and in vitro evidence of Trypanosoma-induced B cell death.Tropical animal health and production · 2024Article
- A Flow Cytometry Study of the Binding and Stimulation Potential of InactivatedPathogens (Basel, Switzerland) · 2023Article
- A Review on Microfluidics-Based Impedance Biosensors.Biosensors · 2023Review
- Toward waterborne protozoa detection using sensing technologies.Frontiers in microbiology · 2023Review
- A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes.PloS one · 2022Article
- Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis.Biosensors · 2021Review
- Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics.Lab on a chip · 2021Article
- An Electrokinetically-Driven Microchip for Rapid Entrapment and Detection of Nanovesicles.Micromachines · 2020Article
- Review
- Image-Based Single Cell Sorting Automation in Droplet Microfluidics.Scientific reports · 2020Article
- Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics.Micromachines · 2020Review
- Electrophysiology-based stratification of pancreatic tumorigenicity by label-free single-cell impedance cytometry.Analytica chimica acta · 2020Article
- Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance.Sensors (Basel, Switzerland) · 2019Article
- Dual-neodymium magnet-based microfluidic separation device.Scientific reports · 2019Article
- Microfluidic-based observation of local bacterial density under antimicrobial concentration gradient for rapid antibiotic susceptibility testing.Biomicrofluidics · 2019Article
- Article
- Dielectric characterization ofJournal of the Royal Society, Interface · 2018Article
- Integrating impedance-based growth-rate monitoring into a microfluidic cell culture platform for live-cell microscopy.Microsystems & nanoengineering · 2018Article
- Disposable microfluidic micromixers for effective capture ofJournal of biological engineering · 2018Article
- Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
At present, there are few technologies which enable the detection, identification and viability analysis of protozoan pathogens including Cryptosporidium and/or Giardia at the single (oo)cyst level. We report the use of Microfluidic Impedance Cytometry (MIC) to characterise the AC electrical (impedance) properties of single parasites and demonstrate rapid discrimination based on viability and species. Specifically, MIC was used to identify live and inactive C. parvum oocysts with over 90% certainty, whilst also detecting damaged and/or excysted oocysts. Furthermore, discrimination of Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia, with over 92% certainty was achieved. Enumeration and identification of (oo)cysts can be achieved in a few minutes, which offers a reduction in identification time and labour demands when compared to existing detection methods.
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Registered trials
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