Evidence map›Paper›PMID 28572030›Full record

ArticleExperimental eye research2017

Streamlined duplex live-dead microplate assay for cultured cells.

Bruce A Pfeffer, Steven J Fliesler

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Bruce A PfefferDepartment of Ophthalmology, University at Buffalo-State University of New York (SUNY), Buffalo, NY, USA; SUNY Eye Institute, Buffalo, NY, USA. Electronic address: brucepfe@buffalo.edu.
Steven J FlieslerDepartment of Ophthalmology, University at Buffalo-State University of New York (SUNY), Buffalo, NY, USA; SUNY Eye Institute, Buffalo, NY, USA; Department of Biochemistry and Neuroscience Program, University at Buffalo- State University of New York (SUNY), Buffalo, NY, USA; Research Service, VA Western New York Healthcare System, Buffalo, NY, USA. Electronic address: fliesler@buffalo.edu.
Buffalo State University · USVA Western New York Healthcare System · US

Funding

University of Buffalo Clinical and Translational Science Institute - Supplement SchulyerUL1TR001412 · NCATS · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI MURPHY, TIMOTHY F · 2015 to 2024
$33.8M
ISOPRENOID METABOLISM IN THE RETINAR01EY007361 · NEI · UNIVERSITY OF SOUTH FLORIDA · PI FLIESLER, STEVEN J. · 1988 to 2015
$4.4M
NCATS NIH HHS UL1 TR001412NEI NIH HHS R01 EY007361
6 · The paper itself

Abstract

A duplex fluorescence assay to assess the viability of cells cultured in multi-well plates is described, which can be carried out in the original culture plate using a plate reader, without exchanges of culture or assay medium, or transfer of cells or cell supernatant. The method uses freshly prepared reagents and does not rely on a proprietary, commercially supplied kit. Following experimental treatment, calcein acetoxymethyl ester (CaAM) is added to each well of cultured cells; after 30 min, the fluorescence intensity (emission λ

Indexed as

ATP-Binding Cassette TransportersBenzene DerivativesBiological AssayCell DeathCell LineCells, CulturedCell SurvivalDimethyl SulfoxideFluoresceinsFluorescenceFluorescent DyesHumansMicroscopy, FluorescenceOrganic ChemicalsPhotoreceptor CellsSaponinsATP-Binding Cassette TransportersBenzene Derivativescalcein AMcumene hydroperoxideDimethyl SulfoxideFluoresceinsFluorescent DyesOrganic ChemicalsSaponinsStaurosporineSYTOX Orange dyeCalcein AMCell viability assayFluorescence assayPlate readerRetinal cell lineSytox Orange

Identifiers

PMID28572030
PMCPMC5697708
OpenAlexW2618412365

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.