ArticleBMC genomics2017
Comparison of molecular serotyping approaches of Streptococcus agalactiae from genomic sequences.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Structure-Informed Prioritization of Phytochemical Antistreptococcal Candidates: Integrated Molecular Docking and In Vitro Proof-of-Concept Validation AgainstCurrent issues in molecular biology · 2026Article
- Evidence of a novel sublineage ofMicrobial genomics · 2025Article
- Serotype Distribution, Virulence, and Antibiotic Resistance Genomic Characterization of Group B Streptococcus - China, 1998-2024.China CDC weekly · 2025Article
- Predomination of hypervirulent ST283 and genetic diversity of levofloxacin resistance in multidrug-resistant, hypervirulentJournal of medical microbiology · 2025Article
- Genomic insights into the diversity, virulence, and antimicrobial resistance of group BFrontiers in cellular and infection microbiology · 2024Article
- Comparative Analysis of the Molecular Characteristics of Group BAnnals of laboratory medicine · 2023Article
- Bacteriocin ProducingMicroorganisms · 2022Article
- Identifying large-scale recombination and capsular switching events inMicrobial genomics · 2022Article
- Article
- GBS-SBG - GBS Serotyping by Genome Sequencing.Microbial genomics · 2021Article
- Potential for Phages in the Treatment of Bacterial Sexually Transmitted Infections.Antibiotics (Basel, Switzerland) · 2021Review
- Phylogeny, recombination, and invasiveness of group B Streptococcus revealed by genomic comparisons of its global strains.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Molecular Identification of Invasive Non-typeable Group BFrontiers in cellular and infection microbiology · 2021Article
- Genomic and phenotypic characterisation of invasive neonatal and colonising group B Streptococcus isolates from Slovenia, 2001-2018.BMC infectious diseases · 2020Article
- Identification of Group B Streptococcus Serotypes and Genotypes in Late Pregnant Women and Neonates That Are Associated With Neonatal Early-Onset Infection in a South China Population.Frontiers in pediatrics · 2020Article
- Antibiotic Resistance and Molecular Epidemiological Characteristics ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2020Article
- Streptococcus agalactiae in pregnant women in Brazil: prevalence, serotypes, and antibiotic resistance.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Review
- Uncertainties in Screening and Prevention of Group B Streptococcus Disease.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Genomic characterisation of perinatal Western Australian Streptococcus agalactiae isolates.PloS one · 2019Article
- Genomic Insights Into the Distribution and Evolution of Group B Streptococcus.Frontiers in microbiology · 2019Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGroup B streptococcus (GBS) capsular polysaccharide is one of the major virulence factors underlying invasive GBS disease and a component of forthcoming vaccines. Serotype classification of GBS is based on the capsule polysaccharide of which ten variants are known to exist (Ia, Ib, II-IX). Current methods for GBS serotype assignment rely on latex agglutination or PCR while more recently a whole genome sequencing method was reported. In this study, three distinct algorithms for serotype assignment from genomic data were assessed using a panel of 790 clinical isolates.
methodsThe first approach utilised the entire capsular locus coupled with a mapping methodology. The second approach continues from the first and utilised a SNP-based methodology across the conserved cpsD-G region to differentiate serotypes Ia-VII and IX. Finally the third approach used the variable cpsG -K region coupled with a mapping methodology. All three approaches were assessed for typeability (percentage of isolates assigned a serotype) and concordance to the latex agglutination methodology.
resultsFollowing comparisons, the third approach using the variable cpsG-K region demonstrated the best performance with 99.9% typeability and 86.7% concordance. Overall, of the 105 discordant isolates, 71 were resolved following retesting of latex agglutination and whole genome sequencing, 20 failed to assign a serotype using latex agglutination and only 14 were found to be truly discordant on re-testing. Comparison of this final approach with the previously described assembly-based approach returned identical results.
conclusionsThese results demonstrated that molecular capsular typing using whole genome sequencing and a mapping-based approach is a viable alternative to the traditional, latex agglutination-based serotyping method and can be implemented in a public health microbiology setting.
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