ArticleACS synthetic biology2017
Multiplex Genome Editing by Natural Transformation (MuGENT) for Synthetic Biology in Vibrio natriegens.
Article in ACS synthetic biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed.
- Is faster always better? A critical assessment of Vibrio natriegens as microbial host for recombinant protein production.Bioprocess and biosystems engineering · 2026Review
- Formate-driven efficient pyruvate biosynthesis inSynthetic and systems biotechnology · 2026Article
- Amplification of computational power by the multiplication of bacteria exploring microfluidic networks encoding mathematical problems.Microsystems & nanoengineering · 2026Article
- Genome shuffling enables quantitative trait locus mapping in Bacillus subtilis.Nature communications · 2026Article
- Low-affinity DNA-binding promotes cooperative activation of natural transformation inJournal of bacteriology · 2026Article
- Nitrogenase regulation inbioRxiv : the preprint server for biology · 2026Article
- Integrating Vibrio natriegens for Photon Manipulation in Living Lighting Devices.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Evaluation of Vibrio natriegens as a fast-growing alternative host for plasmid DNA production.Microbial cell factories · 2026Article
- Mitigating protocatechuic acid toxicity in Vibrio natriegens enables poly-3-hydroxybutyrate production from an aromatic carbon source.Applied microbiology and biotechnology · 2026Article
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- Genetic make-up and regulation of the L-lysine biosynthesis pathway inMicrobial cell (Graz, Austria) · 2026Article
- The tRNA epitranscriptomic landscape and RNA modification enzymes in Vibrio cholerae.PLoS genetics · 2025Article
- ComK-induced cell death is reversed by upregulating the SigB or Spx pathway inMicrobiology spectrum · 2025Article
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- Development of a versatile synthetic microbe for the concurrent degradation of organic pollutants.Advanced biotechnology · 2025Article
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- Deployment of amBio · 2025Article
- Low-biomass pyruvate production with engineered Vibrio natriegens is accompanied by parapyruvate formation.Microbial cell factories · 2025Article
- Two transmembrane transcriptional regulators coordinate to activate chitin-induced natural transformation in Vibrio cholerae.PLoS genetics · 2025Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
Abstract
Vibrio natriegens has recently emerged as an alternative to Escherichia coli for molecular biology and biotechnology, but low-efficiency genetic tools hamper its development. Here, we uncover how to induce natural competence in V. natriegens and describe methods for multiplex genome editing by natural transformation (MuGENT). MuGENT promotes integration of multiple genome edits at high-efficiency on unprecedented time scales. Also, this method allows for generating highly complex mutant populations, which can be exploited for metabolic engineering efforts. As a proof-of-concept, we attempted to enhance production of the value added chemical poly-β-hydroxybutyrate (PHB) in V. natriegens by targeting the expression of nine genes involved in PHB biosynthesis via MuGENT. Within 1 week, we isolated edited strains that produced ∼100 times more PHB than the parent isolate and ∼3.3 times more than a rationally designed strain. Thus, the methods described here should extend the utility of this species for diverse academic and industrial applications.
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