ArticleJournal of visualized experiments : JoVE2017
Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Functional genomics of chitin degradation by Vibrio parahaemolyticus reveals finely integrated metabolic contributions to support environmental fitness.PLoS genetics · 2025Article
- Article
- ZrgA contributes to zinc acquisition in Vibrio parahaemolyticus.Virulence · 2023Article
- Swarming Motility Assays in Salmonella.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Surveying a Swarm: Experimental Techniques To Establish and Examine Bacterial Collective Motion.Applied and environmental microbiology · 2022Article
- Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors inPharmaceutics · 2021Article
- A Trigger Phosphodiesterase Modulates the Global c-di-GMP Pool, Motility, and Biofilm Formation in Vibrio parahaemolyticus.Journal of bacteriology · 2021Article
- Identification of the Flavobacterium johnsoniae cysteate-fatty acyl transferase required for capnine synthesis and for efficient gliding motility.Environmental microbiology · 2021Article
- Antibacterial and Antibiofilm Activities of Chloroindoles AgainstFrontiers in microbiology · 2021Article
- Interdependent Polar Localization of FlhF and FlhG and Their Importance for Flagellum Formation ofFrontiers in microbiology · 2021Article
- Transcriptional regulation by σ factor phosphorylation in bacteria.Nature microbiology · 2020Article
- Chemotaxis arrays inJournal of bacteriology · 2018Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to study the intracellular localization of proteins is essential for the understanding of many cellular processes. In turn, this requires the ability to obtain single cells for fluorescence microscopy, which can be particularly challenging when imaging cells that exist within bacterial communities. For example, the human pathogen Vibrio parahaemolyticus exists as short rod-shaped swimmer cells in liquid conditions that upon surface contact differentiate into a subpopulation of highly elongated swarmer cells specialized for growth on solid surfaces. This paper presents a method to perform single cell fluorescence microscopy analysis of V. parahaemolyticus in its two differential states. This protocol very reproducibly induces differentiation of V. parahaemolyticus into a swarmer cell life-cycle and facilitates their proliferation over solid surfaces. The method produces flares of differentiated swarmer cells extending from the edge of the swarm-colony. Notably, at the very tip of the swarm-flares, swarmer cells exist in a single layer of cells, which allows for their easy transfer to a microscope slide and subsequent fluorescence microscopy imaging of single cells. Additionally, the workflow of image analysis for demographic representation of bacterial societies is presented. As a proof of principle, the analysis of the intracellular localization of chemotaxis signaling arrays in swimmer and swarmer cells of V. parahaemolyticus is described.
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Registered trials
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