ArticleJournal of visualized experiments : JoVE2017
En Face Preparation of Mouse Blood Vessels.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Endothelial cannabinoid CB1 receptor deficiency reduces shear stress-induced arterial inflammation and lipid uptake.Nature communications · 2026Article
- Protocol for tyramide signal amplification immunohistochemical detection of Notch1 signaling in the vascular system.STAR protocols · 2024Article
- High wall shear stress-dependent podosome formation in a novel murine model of intracranial aneurysm.Frontiers in stroke · 2024Article
- Blood flow patterns switch VEGFR2 activity through differential S-nitrosylation and S-oxidation.Cell reports · 2023Article
- Metformin reduces the risk of developing influenza A virus related cardiovascular disease.Heliyon · 2023Article
- Multilineage commitment of Sca-1Theranostics · 2023Article
- Endothelial activation and fibrotic changes are impeded by laminar flow-induced CHK1-SENP2 activity through mechanisms distinct from endothelial-to-mesenchymal cell transition.Frontiers in cardiovascular medicine · 2023Article
- Chromatin condensation regulates endothelial cell adaptation to shear stress.Molecular biology of the cell · 2022Article
- Three-Dimensional Visualization of Atherosclerotic Vessels by Tissue Clearing and Light-Sheet Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- 3D Tissue-Engineered Vascular Drug Screening Platforms: Promise and Considerations.Frontiers in cardiovascular medicine · 2022Review
- Telomerase therapy reverses vascular senescence and extends lifespan in progeria mice.European heart journal · 2021Article
- 3D Bioprinted Multicellular Vascular Models.Advanced healthcare materials · 2021Article
- The subcellular redistribution of NLRC5 promotes angiogenesis via interacting with STAT3 in endothelial cells.Theranostics · 2021Article
- Distinct Effects of Milk-Derived and Fermented Dairy Protein on Gut Microbiota and Cardiometabolic Markers in Diet-Induced Obese Mice.The Journal of nutrition · 2020Article
- Impairment of Flow-Sensitive Inwardly Rectifying KArteriosclerosis, thrombosis, and vascular biology · 2020Article
- Endothelial senescence is induced by phosphorylation and nuclear export of telomeric repeat binding factor 2-interacting protein.JCI insight · 2019Article
- Article
- Pro-atherosclerotic disturbed flow disrupts caveolin-1 expression, localization, and function via glycocalyx degradation.Journal of translational medicine · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Sections of paraffin embedded tissues are routinely used for studying tissue histology and histopathology. However, it is difficult to determine what the three-dimensional tissue morphology is from such sections. In addition, the sections of tissues examined may not contain the region within the tissue that is necessary for the purpose of the ongoing study. This latter limitation hinders histopathological studies of blood vessels since vascular lesions develop in a focalized manner. This requires a method that enables us to survey a wide area of the blood vessel wall, from its surface to deeper regions. A whole mount en face preparation of blood vessels fulfills this requirement. In this article, we will demonstrate how to make en face preparations of the mouse aorta and carotid artery and to immunofluorescently stain them for confocal microscopy and other types of fluorescence-based imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.