ArticleMalaria journal2017
Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene.
Article in Malaria journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Systematic review of sporozoite infection rate of Anopheles mosquitoes in Ethiopia, 2001-2021.Parasites & vectors · 2023Pooled it
- Baseline malaria burden and pyrethroid resistance in Muheza, Tanzania informing a cluster randomized trial of the 3D window screens.Scientific reports · 2026Trial
- Efficacy of a Spatial Repellent for Control of Malaria in Indonesia: A Cluster-Randomized Controlled Trial.The American journal of tropical medicine and hygiene · 2020Trial
- Changing Species Dynamics and Species-Specific Associations Observed between Anopheles and Plasmodium Genera in Diebougou Health District, Southwest Burkina Faso.The American journal of tropical medicine and hygiene · 2026Article
- A multiplex assay to detect mosquito species, bloodmeal host source and Plasmodium in malaria vectors using Nanopore amplicon sequencing.Medical and veterinary entomology · 2026Article
- Anopheles gambiae biting behavior and malaria transmission risk in Simiw, a community located in the coastal forest zone of the Central Region of Ghana.Malaria journal · 2026Article
- Composition, blood meal sources, and entomological inoculation rates of Anopheles mosquitoes in an urban setting, central Ethiopia.Malaria journal · 2026Article
- Spatial temporal distribution ofGigaByte (Hong Kong, China) · 2026Article
- Coustani Group mosquito (Diptera: Culicidae) diversity and malaria transmission in the Democratic Republic of the Congo.Malaria journal · 2025Article
- Urban malaria in Accra, Ghana: transmission risk and influence of irrigated vegetable farms.BMC infectious diseases · 2025Article
- Malaria vector diversity, transmission and insecticide resistance in Island communities along the Volta lake in Southern Ghana.BMC infectious diseases · 2025Article
- Entomological Profiles of Households in Plasmodium falciparum Case Foci and Comparison Areas in Grand'Anse, Haiti.The American journal of tropical medicine and hygiene · 2025Article
- Malaria transmission risk in the city of Accra, Ghana: vector behavior and distribution.Research square · 2025Article
- MALDI-TOF MS for malaria vector surveillance: A cost-comparison analysis using a decision-tree approach.PloS one · 2025Article
- Co-occurrence of native and invasive malaria vectors in anthropogenic habitats in Metehara, Ethiopia: Opportunities for urban malaria control.Current research in parasitology & vector-borne diseases · 2025Article
- Correction: Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene.Malaria journal · 2024Article
- Pathogen prospecting of museums: Reconstructing malaria epidemiology.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Spatiotemporal distribution and bionomics of Anopheles stephensi in different eco-epidemiological settings in Ethiopia.Parasites & vectors · 2024Article
- Assessment of Culicidae collection methods for xenomonitoring lymphatic filariasis in malaria co-infection context in Burkina Faso.PLoS neglected tropical diseases · 2024Article
- Dynamics of malaria vector composition and Plasmodium falciparum infection in mainland Tanzania: 2017-2021 data from the national malaria vector entomological surveillance.Malaria journal · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMolecular tools for detecting malaria-infected mosquitoes with improved practicality, sensitivity and specificity, and high-throughput are required. A common PCR technique used to detect mosquitoes infected with Plasmodium spp. is a nested PCR assay based on the 18s-rRNA gene. However, this technique has several technical limitations, is laborious and time consuming.
methodsIn this study, a PCR-based on the Plasmodium cytochrome oxidase I (COX-I) gene was compared with the 18s-rRNA nested PCR using serial dilutions (330-0.0012 pg) of DNA from Plasmodium vivax, Plasmodium falciparum and Plasmodium knowlesi and with DNA from 48 positive and negative Kenyan mosquitoes (previously detected by using both ELISA and PCR). This assay for Plasmodium spp. DNA detection using the fast COX-I PCR assay was then performed individually on 2122 field collected mosquitoes (from the Solomon Islands) in which DNA was extracted from head and thorax.
resultsThe fast COX-I PCR assay took 1 h to run and consistently detected as low as to 0.043 pg of parasite DNA (equivalent to two parasites) in a single PCR, while analyses with the 18s-rRNA nested PCR required 4 h to complete with a consistent detection threshold of 1.5 pg of DNA. Both assays produced concordant results when applied to the 48 Kenyan control samples with known Plasmodium spp. infection status. The fast COX-I PCR identified 23/2122 Plasmodium-infected mosquitoes from the Solomon Islands.
conclusionsThis new COX-I PCR adapted for a single PCR reaction is a faster, simpler, cheaper, more sensitive technique amenable to high-throughput analyses for Plasmodium DNA detection in mosquitoes and is comparable to the 18s-rRNA nested PCR. The improved sensitivity seen with the fast COX-I PCR will improve the accuracy of mosquito infection rate determination.
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