Article3 Biotech2017
Production, purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juice.
Article in 3 Biotech, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Novel Antarctic Endo-Polygalacturonase for Pectin Extraction and Vegetal Tissue Maceration at Mild Temperatures.Applied biochemistry and biotechnology · 2025Article
- Purification, biochemical characterization, and biotechnological applications of a multifunctional enzyme from the Thermoascus aurantiacus PI3S3 strain.Scientific reports · 2024Article
- The postbiotic potential ofFrontiers in microbiology · 2024Review
- Fungal pectinases: an insight into production, innovations and applications.World journal of microbiology & biotechnology · 2023Review
- Current Insights in Fungal Importance-A Comprehensive Review.Microorganisms · 2023Review
- Heterologous Expression and Characterization of a Novel Exo-Polygalacturonase fromJournal of microbiology and biotechnology · 2023Article
- Pectinase from Microorganisms and Its Industrial Applications.TheScientificWorldJournal · 2022Review
- Pectinolytic lyases: a comprehensive review of sources, category, property, structure, and catalytic mechanism of pectate lyases and pectin lyases.Bioresources and bioprocessing · 2021Review
- Method for the Production and Purification of Plant Immuno-Active Xylanase fromInternational journal of molecular sciences · 2021Article
- Partial Purification and Characterisation of Pectinase Produced byTropical life sciences research · 2021Article
- Conversion of Exhausted Sugar Beet Pulp into Fermentable Sugars from a Biorefinery Approach.Foods (Basel, Switzerland) · 2020Article
- Isolation and physicochemical property of individual parenchyma cells from mealy sweet potato.Food science and biotechnology · 2020Article
- Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing.Frontiers in nutrition · 2020Review
- Pectinolytic enzymes-solid state fermentation, assay methods and applications in fruit juice industries: a review.3 Biotech · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polygalacturonases (PG) represent an important member of pectinases group of enzymes with immense industrial applications. A fungal strain Aspergillus niger MTCC478 was used for the production of polygalacturonase both under submerged and solid-state fermentation condition. Further its production was optimized under solid-state fermentation condition with media comprising of wheat bran and tea extract. Purification of an exo-PG was achieved by acetone precipitation (60-90%) and CM-cellulose column chromatography revealing 15.28-fold purification with a specific activity of 33.47 U/mg protein and 1.2% yield. A relative molecular mass of purified PG was approximately 124.0 kDa. The pH and temperature optimum was found to be 4 and 50 °C, respectively. The k
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