ArticleScientific reports2017
Building early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- Assessment of Ubiquitous Promoters Driving Fluorescent Marker and Transposase Expression to Develop a High-PerformanceInsects · 2026Article
- Genetic and behavioral analyses suggest that larval and adult stages of Lucilia cuprina employ different sensory systems to detect rotten beef.Parasites & vectors · 2025Article
- Tetracycline has no long-term effects on gut physiology and microbiome of the new world screwworm, Cochliomyia hominivorax, which has positive implications for transgenic male-only rearing systems.G3 (Bethesda, Md.) · 2025Article
- Effects of antibiotics on theFrontiers in bioengineering and biotechnology · 2023Article
- Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.PLoS genetics · 2021Article
- An early female lethal system of the New World screwworm, Cochliomyia hominivorax, for biotechnology-enhanced SIT.BMC genetics · 2020Article
- Identification and characterization of four Drosophila suzukii cellularization genes and their promoters.BMC genetics · 2020Article
- Building a transgenic sexing strain for genetic control of the Australian sheep blow fly Lucilia cuprina using two lethal effectors.BMC genetics · 2020Article
- Using Moderate Transgene Expression to Improve the Genetic Sexing System of the Australian Sheep Blow FlyInsects · 2020Article
- Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males.PLoS genetics · 2020Article
- Genetic breakdown of a Tet-off conditional lethality system for insect population control.Nature communications · 2020Article
- Transcriptome Analysis of the Oriental Fruit FlyInsects · 2020Article
- Improved transgenic sexing strains for genetic control of the Australian sheep blow fly Lucilia cuprina using embryo-specific gene promoters.Molecular genetics and genomics : MGG · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic sexing strains (TSS) that carry conditional female lethal genes are advantageous for genetic control programs based on the sterile insect technique (SIT). It is desirable if females die early in development as larval diet is a major cost for mass production facilities. This can be achieved by using a gene promoter that is only active in embryos to drive expression of the tetracycline transactivator (tTA), the transcription factor commonly used in two-component TSS. While an embryo-specific promoter is ideal it may not be essential for assembling an effective TSS as tTA can be repressed by addition of tetracycline to the diet at larval and/or adult stages. Here we have investigated this idea by isolating and employing the promoters from the Lucilia spitting image and actin 5C genes to drive tTA expression in embryos and later stages. L. cuprina TSS with the tTA drivers and tTA-regulated tetO-Lshid effectors produced only females when raised on a limited tetracycline diet. The Lshid transgene contains a sex-specific intron and as a consequence only females produce LsHID protein. TSS females died at early larval stages, which makes the lines advantageous for an SIT program.
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