ArticleScientific reports2017
Production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 127 citations in OpenAlex.
- Trial
- Metabolic engineering ofSynthetic and systems biotechnology · 2026Article
- High-yield production of hydroxytyrosol through metabolic engineering ofSynthetic and systems biotechnology · 2026Article
- Systems metabolic engineering for hydroxytyrosol production inApplied and environmental microbiology · 2026Article
- Quality differentiation and salidroside biosynthesis inFrontiers in plant science · 2026Article
- Tailoring Regioselectivity-Controlled UDP-Glycosyltransferase for Bidirectional Glycosylation of Tyrosol via Free Energy-Driven Pocket Reshaping and Tunnel Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Efficient synthesis of salidroside using mined glycosyltransferase through cascade reaction.Bioprocess and biosystems engineering · 2025Article
- Structure-based virtual screening aids the identification of glycosyltransferases in the biosynthesis of salidroside.Plant biotechnology journal · 2025Article
- Transcriptome analysis and reverse engineering verification of SNZ3Biotechnology for biofuels and bioproducts · 2025Article
- Production of aromatic amino acids and their derivatives by Escherichia coli and Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Review
- Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli.Scientific reports · 2025Article
- Semi-rational design and modification of phosphoketolase to improve the yield of tyrosol inSynthetic and systems biotechnology · 2025Article
- Salidroside: An Overview of Its Promising Potential and Diverse Applications.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Foliar Nutrition Strategies for Enhancing Phenolic and Amino Acid Content in Olive Leaves.Plants (Basel, Switzerland) · 2024Article
- Hydroxytyrosol isolation, comparison of synthetic routes and potential biological activities.Food science & nutrition · 2024Review
- Distribution, biosynthesis, and synthetic biology of phenylethanoid glycosides in the order Lamiales.Plant biotechnology (Tokyo, Japan) · 2024Article
- Biosynthesis and Biotechnological Synthesis of Hydroxytyrosol.Foods (Basel, Switzerland) · 2024Review
- Efficient Biosynthesis of Salidroside via ArtificialACS omega · 2024Article
- Plant-Derived Caffeic Acid and Its Derivatives: An Overview of Their NMR Data and Biosynthetic Pathways.Molecules (Basel, Switzerland) · 2024Review
- Screening of Secondary Metabolites Produced byMolecules (Basel, Switzerland) · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyphenols, which include phenolic acids, flavonoids, stilbenes, and phenylethanoids, are generally known as useful antioxidants. Tyrosol, hydroxytyrosol, and salidroside are typical phenylethanoids. Phenylethanoids are found in plants such as olive, green tea, and Rhodiola and have various biological activities, including the prevention of cardiovascular diseases, cancer, and brain damage. We used Escherichia coli to synthesize three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside. To synthesize tyrosol, the aromatic aldehyde synthase (AAS) was expressed in E. coli. Hydroxytyrosol was synthesized using E. coli harboring AAS and HpaBC, which encodes hydroxylase. In order to synthesize salidroside, 12 uridine diphosphate-dependent glycosyltransferases (UGTs) were screened and UGT85A1 was found to convert tyrosol to salidroside. Using E. coli harboring AAS and UGT85A1, salidroside was synthesized. Through the optimization of these three E. coli strains, we were able to synthesize 531 mg/L tyrosol, 208 mg/L hydroxytyrosol, and 288 mg/L salidroside, respectively.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.