Evidence map›Paper›PMID 28564609›Full record

ArticleCell reports2017

Sequential Steps of CRAC Channel Activation.

Raz Palty, Zhu Fu, Ehud Y Isacoff

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. The Orai Pore Opening Mechanism.International journal of molecular sciences · 2021
    Review
  7. Review
  8. Store-Operated Calcium Channels: From Function to Structure and Back Again.Cold Spring Harbor perspectives in biology · 2020
    Review
  9. Review
  10. Article
  11. Review
  12. Review
  13. STIM1 activation of Orai1.Cell calcium · 2019
    Review
  14. Article
  15. Article
  16. Article
  17. CaThe Journal of physiology · 2018
    Review
  18. Mapping the functional anatomy of Orai1 transmembrane domains for CRAC channel gating.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Raz PaltyDepartment of Biochemistry, Ruth and Bruce Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa 31096, Israel. Electronic address: razpalty@technion.ac.il.
Zhu FuDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Ehud Y IsacoffDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Bioscience Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: ehud@berkeley.edu.
University of California, Berkeley · USTechnion – Israel Institute of Technology · IL

Funding

NDC for the Optical Control of Biological FunctionPN2EY018241 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI ISACOFF, EHUD · 2006 to 2015
$37.5M
STRUCTURAL REARRANGEMENTS IN CHANNEL GATINGR01NS035549 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI ISACOFF, EHUD · 1996 to 2013
$4.1M
Novel tools for cell-specific imaging of functional connectivity and circuit operationsU01MH109069 · NIMH · UNIVERSITY OF CALIFORNIA BERKELEY · PI ISACOFF, EHUD · 2015 to 2017
$2.2M
Voltage Gating MechanismsR01GM117051 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI ISACOFF, EHUD · 2016 to 2019
$1.3M
NEI NIH HHS PN2 EY018241NIGMS NIH HHS R01 GM117051NIMH NIH HHS U01 MH109069NINDS NIH HHS R01 NS035549
6 · The paper itself

Abstract

Interaction between the endoplasmic reticulum protein STIM1 and the plasma membrane channel ORAI1 generates calcium signals that are central for diverse cellular functions. How STIM1 binds and activates ORAI1 remains poorly understood. Using electrophysiological, optical, and biochemical techniques, we examined the effects of mutations in the STIM1-ORAI1 activating region (SOAR) of STIM1. We find that SOAR mutants that are deficient in binding to resting ORAI1 channels are able to bind to and boost activation of partially activated ORAI1 channels. We further show that the STIM1 binding regions on ORAI1 undergo structural rearrangement during channel activation. The results suggest that activation of ORAI1 by SOAR occurs in multiple steps. In the first step, SOAR binds to ORAI1, partially activates the channel, and induces a rearrangement in the SOAR-binding site of ORAI1. That rearrangement of ORAI1 then permits sequential steps of SOAR binding, via distinct molecular interactions, to fully activate the channel.

Indexed as

Ion Channel GatingBinding SitesCalciumCalcium ChannelsCytosolHEK293 CellsHumansLigandsMutant ProteinsMutationORAI1 ProteinProtein BindingProtein ConformationStromal Interaction Molecule 1CalciumCalcium ChannelsLigandsMutant ProteinsORAI1 ProteinStromal Interaction Molecule 1calciumCRAC channelgatingORAI1STIM1

Identifiers

PMID28564609
PMCPMC5519287
OpenAlexW2620225514

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.