ArticlePloS one2017
Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- Functional analysis of a rice 12-oxo-phytodienoic acid reductase gene (OsOPR1) involved in Cd stress tolerance.Molecular biology reports · 2024Article
- Accumulation and Enrichment of Trace Elements by Yeast Cells and Their Applications: A Critical Review.Microorganisms · 2022Review
- Mycoremediation of heavy metals: processes, mechanisms, and affecting factors.Environmental science and pollution research international · 2021Review
- Review
- Mechanisms of Copper Tolerance, Accumulation, and Detoxification in the Marine MacroalgaPlants (Basel, Switzerland) · 2020Review
- Microalgal Metallothioneins and Phytochelatins and Their Potential Use in Bioremediation.Frontiers in microbiology · 2020Review
- Accumulation of Ag(I) by Saccharomyces cerevisiae Cells Expressing Plant Metallothioneins.Cells · 2018Article
- Heavy Metal Removal by Bioaccumulation Using Genetically Engineered Microorganisms.Frontiers in bioengineering and biotechnology · 2018Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we engineered yeast cells armed for heavy metal accumulation by targeting plant metallothioneins to the inner face of the yeast plasma membrane. Metallothioneins (MTs) are cysteine-rich proteins involved in the buffering of excess metal ions, especially Cu(I), Zn(II) or Cd(II). The cDNAs of seven Arabidopsis thaliana MTs (AtMT1a, AtMT1c, AtMT2a, AtMT2b, AtMT3, AtMT4a and AtMT4b) and four Noccaea caerulescens MTs (NcMT1, NcMT2a, NcMT2b and NcMT3) were each translationally fused to the C-terminus of a myristoylation green fluorescent protein variant (myrGFP) and expressed in Saccharomyces cerevisiae cells. The myrGFP cassette introduced a yeast myristoylation sequence which allowed directional targeting to the cytosolic face of the plasma membrane along with direct monitoring of the intracellular localization of the recombinant protein by fluorescence microscopy. The yeast strains expressing plant MTs were investigated against an array of heavy metals in order to identify strains which exhibit the (hyper)accumulation phenotype without developing toxicity symptoms. Among the transgenic strains which could accumulate Cu(II), Zn(II) or Cd(II), but also non-canonical metal ions, such as Co(II), Mn(II) or Ni(II), myrGFP-NcMT3 qualified as the best candidate for bioremediation applications, thanks to the robust growth accompanied by significant accumulative capacity.
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