ArticleTissue engineering. Part C, Methods2017
Optical Method to Quantify Mechanical Contraction and Calcium Transients of Human Pluripotent Stem Cell-Derived Cardiomyocytes.
Article in Tissue engineering. Part C, Methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.npj biomedical innovations · 2026Article
- Disruption of ATP Synthase Spatiotemporal Organization, CaAging cell · 2026Article
- Noninvasive Quantification of Contractile Dynamics in Cardiac Cells, Spheroids, and Organs-on-a-Chip Using High-Frequency Ultrasound.ACS nano · 2024Article
- Micropatterned fibrin scaffolds increase cardiomyocyte alignment and contractility for the fabrication of engineered myocardial tissue.Journal of biomedical materials research. Part A · 2023Article
- Flexible 3D printed microwires and 3D microelectrodes for heart-on-a-chip engineering.Biofabrication · 2023Article
- Simultaneous Widefield Voltage and Dye-Free Optical Mapping Quantifies Electromechanical Waves in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.ACS photonics · 2023Article
- Microengineered platforms for characterizing the contractile function of in vitro cardiac models.Microsystems & nanoengineering · 2022Review
- Creation of a contractile biomaterial from a decellularized spinach leaf without ECM protein coating: An in vitro study.Journal of biomedical materials research. Part A · 2020Article
- Mutation-specific differences in arrhythmias and drug responses in CPVT patients: simultaneous patch clamp and video imaging of iPSC derived cardiomyocytes.Molecular biology reports · 2020Article
- Cardiomyocyte mechanodynamics under conditions of actin remodelling.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Modeling cardiac complexity: Advancements in myocardial models and analytical techniques for physiological investigation and therapeutic developmentAPL bioengineering · 2019Review
- Considerations for anFrontiers in pharmacology · 2019Review
- An Ultrasensitive Calcium Reporter System via CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells.iScience · 2018Article
- Multi-parametric cell profiling with a CMOS quad-modality cellular interfacing array for label-free fully automated drug screening.Lab on a chip · 2018Article
- Development of a Contractile Cardiac Fiber From Pluripotent Stem Cell Derived Cardiomyocytes.Frontiers in cardiovascular medicine · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Differentiation of human pluripotent stem cells into cardiomyocytes (hPS-CMs) holds promise for myocardial regeneration therapies, drug discovery, and models of cardiac disease. Potential cardiotoxicities may affect hPS-CM mechanical contraction independent of calcium signaling. Herein, a method using an image capture system is described to measure hPS-CM contractility and intracellular calcium concurrently, with high spatial and temporal resolution. The image capture system rapidly alternates between brightfield and epifluorescent illumination of contracting cells. Mechanical contraction is quantified by a speckle tracking algorithm applied to brightfield image pairs, whereas calcium transients are measured by a fluorescent calcium reporter. This technique captured changes in contractile strain, calcium transients, and beat frequency of hPS-CMs over 21 days in culture, as well as acute responses to isoproterenol and Cytochalasin D. The technique described above can be applied without the need to alter the culture platform, allowing for determination of hPS-CM behavior over weeks in culture for drug discovery and myocardial regeneration applications.
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