ArticleAnalytical chemistry2017
Toward a Droplet-Based Single-Cell Radiometric Assay.
Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Multiplex fluorescence detection of single-cell droplet microfluidics and its application in quantifying protein expression levels.Biomicrofluidics · 2023Article
- Particle Counting Methods Based on Microfluidic Devices.Micromachines · 2023Review
- Recent Technical Advances in Accelerating the Clinical Translation of Small Animal Brain Imaging: Hybrid Imaging, Deep Learning, and Transcriptomics.Frontiers in medicine · 2022Review
- Flow radiocytometry using droplet optofluidics.Biosensors & bioelectronics · 2021Article
- Microfluidics-Coupled Radioluminescence Microscopy forAnalytical chemistry · 2021Article
- Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments.Science advances · 2020Article
- Article
- Chemical operations on a living single cell by open microfluidics for wound repair studies and organelle transport analysis.Chemical science · 2019Article
- Radio-Fluorogenic Gel Dosimetry with Coumarin.Bioengineering (Basel, Switzerland) · 2018Article
- Development and characterization of a scintillating cell imaging dish for radioluminescence microscopy.The Analyst · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Radiotracers are widely used to track molecular processes, both in vitro and in vivo, with high sensitivity and specificity. However, most radionuclide detection methods have spatial resolution inadequate for single-cell analysis. A few existing methods can extract single-cell information from radioactive decays, but the stochastic nature of the process precludes high-throughput measurement (and sorting) of single cells. In this work, we introduce a new concept for translating radioactive decays occurring stochastically within radiolabeled single-cells into an integrated, long-lasting fluorescence signal. Single cells are encapsulated in radiofluorogenic droplets containing molecular probes sensitive to byproducts of ionizing radiation (primarily reactive oxygen species, or ROS). Different probes were examined in bulk solutions, and dihydrorhodamine 123 (DHRh 123) was selected as the lead candidate due to its sensitivity and reproducibility. Fluorescence intensity of DHRh 123 in bulk increased at a rate of 54% per Gy of X-ray radiation and 15% per MBq/ml of 2-deoxy-2-[
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.