ArticleJournal of food science and technology2017
Two isoforms of lipoxygenase from mature grains of pearl millet [
Article in Journal of food science and technology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 19 citations in OpenAlex.
- A trade-off between shelf-life mitigation and nutritional retention: a persistent challenge in pearl millet.Frontiers in nutrition · 2026Review
- Enhancing extracellular production of lipoxygenase in Escherichia coli by signal peptides and autolysis system.Microbial cell factories · 2022Article
- Combinatorial strategy towards the efficient expression of lipoxygenase in Escherichia coli at elevated temperatures.Applied microbiology and biotechnology · 2020Article
- Purification and Product Characterization of Lipoxygenase from Opium Poppy Cultures (Molecules (Basel, Switzerland) · 2019Article
- HPLC Analysis and Biochemical Characterization of LOX from Eschscholtzia californica Cham.Molecules (Basel, Switzerland) · 2017Article
- Shelf life determinants and enzyme activities of pearl millet: a comparison of changes in stored flour of hybrids, CMS lines, inbreds and composites.Journal of food science and technology · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
This study describes the partial purification and characterization of lipoxygenase (LOX) from pearl millet mature grains of inbred HBL 0843-2. Two isoforms of LOX, i.e., LOX 1 and LOX 2, were purified using ammonium sulphate fractionation, gel filtration chromatography and ion exchange chromatography near homogeneity to 56 and 40 folds with yield of 28 and 24%, respectively. LOX 1 and LOX 2 having molecular masses of approximately 85 and 79 kDa, respectively were purified. LOX 1 and LOX 2 exhibited maximum activity at pH 4.5 and 4.8, respectively at 25 °C temperature. Both the isoforms, which showed thermostability up to 35 °C when incubated for 30 min, were stable at a pH range of 7-7.8. LOX 1 and LOX 2 had apparent
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