Evidence map›Paper›PMID 28559166›Full record

ArticleJournal of structural biology2017

Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM.

Jie He, Chyongere Hsieh, Yongping Wu, Thomas Schmelzer, Pan Wang, Ying Lin, Michael Marko, Haixin Sui

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of structural biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jie HeWadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201, USA.
Chyongere HsiehWadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201, USA.
Yongping WuWadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201, USA; Zhejiang Agriculture and Forestry University, Zhejiang Province, PR China.
Thomas SchmelzerTGS Technologies, 702 Little Creek Lane, Cranberry Township, PA 16066, USA.
Pan WangSchool of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
Ying LinSchool of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
Michael MarkoWadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201, USA.
Haixin SuiWadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201, USA; Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, NY 12201, USA. Electronic address: haixin.sui@health.ny.gov.

Funding

TECHNOLOGY DEVELOPMENT FOR CRYO-EM APPLICATIONSR35GM118023 · NIGMS · WADSWORTH CENTER · PI MARKO, MICHAEL · 2016 to 2020
$3.2M
Focused Ion Beam Milling for Cryo-electron TomographyR01GM097010 · NIGMS · WADSWORTH CENTER · PI MARKO, MICHAEL, SUI, HAIXIN · 2011 to 2015
$2.8M
Structure and Function of Primary CiliaR01GM101026 · NIGMS · WADSWORTH CENTER · PI SUI, HAIXIN · 2013 to 2017
$1.4M
NIGMS NIH HHS R01 GM097010NIGMS NIH HHS R01 GM101026NIGMS NIH HHS R35 GM118023
6 · The paper itself

Abstract

Cryo-electron tomography (cryo-ET) is a well-established technique for studying 3D structural details of subcellular macromolecular complexes and organelles in their nearly native context in the cell. A primary limitation of the application of cryo-ET is the accessible specimen thickness, which is less than the diameters of almost all eukaryotic cells. It has been shown that focused ion beam (FIB) milling can be used to prepare thin, distortion-free lamellae of frozen biological material for high-resolution cryo-ET. Commercial cryosystems are available for cryo-FIB specimen preparation, however re-engineering and additional fixtures are often essential for reliable results with a particular cryo-FIB and cryo-transmission electron microscope (cryo-TEM). Here, we describe our optimized protocol and modified instrumentation for cryo-FIB milling to produce thin lamellae and subsequent damage-free cryotransfer of the lamellae into our cartridge-type cryo-TEM.

Indexed as

Cryoelectron MicroscopyEquipment DesignFrozen SectionsMethodsMicroscopy, Electron, TransmissionSpecimen HandlingYeastsAlcohol-oxidaseCryo-ETCryo-FIBOrganellesTEM cartridgesYeast

Identifiers

PMID28559166
PMCPMC5555045

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.