ArticleLangmuir : the ACS journal of surfaces and colloids2017
Buffers Strongly Modulate Fibrin Self-Assembly into Fibrous Networks.
Article in Langmuir : the ACS journal of surfaces and colloids, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 55 citations in OpenAlex.
- Impact of acid selection on thrombelastometric test results - an in-vitro cohort study.Thrombosis journal · 2026Article
- Mechanically Spatio-Chimeric Fibrin Assembly Enables Vascular-Integrated Muscle Reconstruction for Volumetric Muscle Loss Repair.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Stable Protein-Based G-Quadruplex-Derived Supramolecular Bioinks as Tunable ECM-Mimetic Constructs Assembled by Combining Non-Covalent and Covalent Strategies.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Bioconvergence of sound-guided and supramolecular assembly strategies to create peptide-protein composite hydrogels with predictable shape-to-function features.Materials today. Bio · 2026Article
- Proteomic Evidence for Amyloidogenic Cross-Seeding in Fibrinaloid Microclots.International journal of molecular sciences · 2024Article
- Laser speckle rheological microscopy reveals wideband viscoelastic spectra of biological tissues.Science advances · 2024Article
- Depletion-Induced Self-Assembly of Colloidal Particles on a Solid Substrate.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Clots reveal anomalous elastic behavior of fiber networks.Science advances · 2024Article
- Microrheology and structural quantification of hypercoagulable clots.Biomedical optics express · 2023Article
- pH and Thrombin Concentration Are Decisive in Synthesizing Stiff, Stable, and Open-Porous Fibrin-Collagen Hydrogel Blends without Chemical Cross-Linker.Advanced healthcare materials · 2023Article
- A comparative high-resolution physicochemical analysis of commercially available fibrin sealants: Impact of sealant osmolality on biological performance.Journal of biomedical materials research. Part A · 2023Article
- Correlation between Fibrin Fibrillation Kinetics and the Resulting Fibrin Network Microstructure.Advanced healthcare materials · 2023Article
- Snake venom-defined fibrin architecture dictates fibroblast survival and differentiation.Nature communications · 2023Article
- Mechanical reinforcement of granular hydrogels.Chemical science · 2022Review
- Increasing salinity of fibrinogen solvent generates stable fibrin hydrogels for cell delivery or tissue engineering.PloS one · 2021Article
- Aggregates Dramatically Alter Fibrin Ultrastructure.Biophysical journal · 2020Article
- Fibrin-based Bioinks: New Tricks from an Old Dog.International journal of bioprinting · 2020Review
- Engineering a Genetically Encoded Magnetic Protein Crystal.Nano letters · 2019Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fibrin is a plasma protein with a central role in blood clotting and wound repair. Upon vascular injury, fibrin forms resilient fibrillar networks (clots) via a multistep self-assembly process, from monomers, to double-stranded protofibrils, to a branched network of thick fibers. In vitro, fibrin self-assembly is sensitive to physicochemical conditions like the solution pH and ionic strength, which tune the strength of the noncovalent driving forces. Here we report a surprising finding that the buffer-which is necessary to control the pH and is typically considered to be inert-also significantly influences fibrin self-assembly. We show by confocal microscopy and quantitative light scattering that various common buffering agents have no effect on the initial assembly of fibrin monomers into protofibrils but strongly hamper the subsequent lateral association of protofibrils into thicker fibers. We further find that the structural changes are independent of the molecular structure of the buffering agents as well as of the activation mechanism and even occur in fibrin networks formed from platelet-poor plasma. This buffer-mediated decrease in protofibril bundling results in a marked reduction in the permeability of fibrin networks but only weakly influences the elastic modulus of fibrin networks, providing a useful tuning parameter to independently control the elastic properties and the permeability of fibrin networks. Our work raises the possibility that fibrin assembly in vivo may be regulated by variations in the acute-phase levels of bicarbonate and phosphate, which act as physiological buffering agents of blood pH. Moreover, our findings add a new example of buffer-induced effects on biomolecular self-assembly to recent findings for a range of proteins and lipids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.