Evidence map›Paper›PMID 28557611›Full record

ArticleNucleus (Austin, Tex.)2017

Lamin A and microtubules collaborate to maintain nuclear morphology.

Zeshan Tariq, Haoyue Zhang, Alexander Chia-Liu, Yang Shen, Yantenew Gete, Zheng-Mei Xiong, Claire Tocheny, Leonard Campanello, Di Wu, Wolfgang Losert and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleus (Austin, Tex.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 61 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Lamin A/C as a biomarker for breast cancer.Molecular biology reports · 2025
    Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Knockdown ofCell adhesion & migration · 2023
    Article
  16. Review
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Zeshan Tariqa Department of Cell Biology and Molecular Genetics , University of Maryland , College Park , MD , USA.
Haoyue Zhanga Department of Cell Biology and Molecular Genetics , University of Maryland , College Park , MD , USA.
Alexander Chia-Liub Department of Physics , University of Maryland , College Park , MD , USA.
Yang Shenb Department of Physics , University of Maryland , College Park , MD , USA.
Yantenew Getea Department of Cell Biology and Molecular Genetics , University of Maryland , College Park , MD , USA.
Zheng-Mei Xionga Department of Cell Biology and Molecular Genetics , University of Maryland , College Park , MD , USA.ORCID http://orcid.org/0000-0001-5104-9064
Claire Tochenyc Department of Biology , The College of William and Mary , Williamsburg , VA , USA.
Leonard Campanellob Department of Physics , University of Maryland , College Park , MD , USA.
Di Wua Department of Cell Biology and Molecular Genetics , University of Maryland , College Park , MD , USA.
Wolfgang Losertb Department of Physics , University of Maryland , College Park , MD , USA.
Kan Caoa Department of Cell Biology and Molecular Genetics , University of Maryland , College Park , MD , USA.
University of Maryland, College Park · USWilliam & Mary · US

Funding

Investigation of smooth muscle cell loss in progeriaR01HL126784 · NHLBI · UNIV OF MARYLAND, COLLEGE PARK · PI CAO, KAN · 2016 to 2020
$2.1M
NHLBI NIH HHS R01 HL126784
6 · The paper itself

Abstract

Lamin A (LA) is a critical structural component of the nuclear lamina. Mutations within the LA gene (LMNA) lead to several human disorders, most striking of which is Hutchinson-Gilford Progeria Syndrome (HGPS), a premature aging disorder. HGPS cells are best characterized by an abnormal nuclear morphology known as nuclear blebbing, which arises due to the accumulation of progerin, a dominant mutant form of LA. The microtubule (MT) network is known to mediate changes in nuclear morphology in the context of specific events such as mitosis, cell polarization, nucleus positioning and cellular migration. What is less understood is the role of the microtubule network in determining nuclear morphology during interphase. In this study, we elucidate the role of the cytoskeleton in regulation and misregulation of nuclear morphology through perturbations of both the lamina and the microtubule network. We found that LA knockout cells exhibit a crescent shape morphology associated with the microtubule-organizing center. Furthermore, this crescent shape ameliorates upon treatment with MT drugs, Nocodazole or Taxol. Expression of progerin, in LA knockout cells also rescues the crescent shape, although the response to Nocodazole or Taxol treatment is altered in comparison to cells expressing LA. Together these results describe a collaborative effort between LA and the MT network to maintain nuclear morphology.

Indexed as

Cell NucleusFluorescent Antibody TechniqueHumansLamin Type AMicroscopy, ConfocalMicrotubulesMutationLamin Type AHGPSlamin Amicrobutublenuclear shapeprogerin

Identifiers

PMID28557611
PMCPMC5597299
OpenAlexW2618393344

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.