Evidence map›Paper›PMID 28556922›Full record

ArticleJournal of microscopy2017

Perceptually accurate display of two greyscale images as a single colour image.

A B Taylor, M S Ioannou, T Watanabe, K Hahn, T-L Chew

Open access · greenAbstract read
In one paragraph

Article in Journal of microscopy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Analysis of facial redness by comparing VISIA and YLGTD.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2023
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

A B TaylorAdvanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, U.S.A.ORCID 0000-0002-4192-2453
M S IoannouAdvanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, U.S.A.
T WatanabeDepartment of Pharmacology, UNC-Chapel Hill School of Medicine, Chapel Hill, North Carolina, U.S.A.
K HahnDepartment of Pharmacology, UNC-Chapel Hill School of Medicine, Chapel Hill, North Carolina, U.S.A.
T-L ChewAdvanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, U.S.A.
Janelia Research Campus · USUniversity of North Carolina at Chapel Hill · US

Funding

INTERACTIVE GRAPHICS FOR MOLECULAR STUDIES &MICROSCOPYP41EB002025 · NIBIB · UNIV OF NORTH CAROLINA CHAPEL HILL · PI SUPERFINE, RICHARD · 2003 to 2019
$16.9M
Spatio-temporal dynamics of GEF-GTPase networksP01GM103723 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI HAHN, KLAUS M. · 2013 to 2017
$5.5M
NIBIB NIH HHS P41 EB002025NIGMS NIH HHS P01 GM103723
6 · The paper itself

Abstract

Life scientists often desire to display the signal from two different molecular probes as a single colour image, so as to convey information about the probes' relative concentrations as well as their spatial corelationship. Traditionally, such colour images are created through a merge display, where each greyscale signal is assigned to different channels of an RGB colour image. However, human perception of colour and greyscale intensity is not equivalent. Thus, a merged image display conveys to the typical viewer only a subset of the absolute and relative intensity information present in and between two greyscale images. The Commission Internationale de l'Eclairage L*a*b* colour space (CIELAB) has been designed to specify colours according to the perceptually defined quantities of hue (perceived colour) and luminosity (perceived brightness). Here, we use the CIELAB colour space to encode two dimensions of information about two greyscale images within these two perceptual dimensions of a single colour image. We term our method a Perceptually Uniform Projection display and show using biological image examples how these displays convey more information about two greyscale signals than comparable RGB colour space-based techniques.

Indexed as

Cytological TechniquesImage Processing, Computer-AssistedMicroscopy, ConfocalMicroscopy, FluorescenceColour spacefluorescence micrographsmerge displayratiometric display

Identifiers

PMID28556922
PMCPMC5637524
OpenAlexW2618307335

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.