ArticleThe British journal of cancer. Supplement1988
Purification of erythroid progenitor cells and characterization of erythropoietin receptors.
Article in The British journal of cancer. Supplement, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 4 citations in OpenAlex.
- Erythropoietin has a mitogenic and positive chemotactic effect on endothelial cells.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Highly purified murine and human erythroid progenitor cells at the colony-forming unit-erythroid (CFU-E) stage of development were prepared and recombinant human erythropoietin (rEp) was radioiodinated with retention of full biological activity. Specific binding of 125I-rEp to the murine cells revealed 950 receptors on the cell surface. Three hundred had a Kd of 0.09 nM while the remaining receptors had a Kd of 0.57 nM. The human erythroid progenitor cells also had two classes of receptors with a similar number per cell and similar distribution. The high affinity receptors had a Kd of 0.15 nM while the remaining receptors had a Kd of 0.37 nM. 125I-rEp was rapidly internalized into the cells at 37 C and metabolized to iodotyrosine. When cross-linking of 125I-rEp to the murine erythroid progenitor cells was performed with disuccinimidyl suberate, two labelled bands of 100 and 85 kDa were demonstrated. The radioactivity of both bands was reduced when binding was performed in the presence of excessive unlabelled rEp indicating a specific interaction of 125I-rEp with the receptor.
Indexed as
Identifiers
2855465PMC2149105W981628214What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.