Evidence map›Paper›PMID 28553947›Full record

ArticleNature chemical biology2017

Structural basis for high-affinity fluorophore binding and activation by RNA Mango.

Robert J Trachman, Natalia A Demeshkina, Matthew W L Lau, Shanker Shyam S Panchapakesan, Sunny C Y Jeng, Peter J Unrau, Adrian R Ferré-D'Amaré

Open access · greenAbstract read
In one paragraph

Article in Nature chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.

0numbers the graph read from it
0cells of the map it votes in
80citing papers in PubMed
7.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

80 citing papers in PubMed, 135 citations in OpenAlex.

  1. Article
  2. Article
  3. Honeydew: A Fluorescent Aptamer for Low-Cost Azo Food Dyes.Chembiochem : a European journal of chemical biology · 2026
    Article
  4. Article
  5. Article
  6. Sequence and ionic requirements of pUG fold quadruplexes.bioRxiv : the preprint server for biology · 2025
    Article
  7. Article
  8. Article
  9. Article
  10. Structural basis and affinity improvement for an ATP-binding DNA aptamer.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Structure-based insights into fluorogenic RNA aptamers.Acta biochimica et biophysica Sinica · 2024
    Review
  18. Review
  19. Structure-based investigation of a DNA aptamer targeting PTK7 reveals an intricate 3D fold guiding functional optimization.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  20. Article

20 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Robert J TrachmanBiochemistry and Biophysics Center, National Heart, Lung and Blood Institute, Bethesda, Maryland, USA.
Natalia A DemeshkinaBiochemistry and Biophysics Center, National Heart, Lung and Blood Institute, Bethesda, Maryland, USA.
Matthew W L LauBiochemistry and Biophysics Center, National Heart, Lung and Blood Institute, Bethesda, Maryland, USA.
Shanker Shyam S PanchapakesanDepartment of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.
Sunny C Y JengDepartment of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.
Peter J UnrauDepartment of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.
Adrian R Ferré-D'AmaréBiochemistry and Biophysics Center, National Heart, Lung and Blood Institute, Bethesda, Maryland, USA.
National Heart Lung and Blood Institute · USSimon Fraser University · CA

Funding

RNA: Structure, Biophysics and PhysiologyZIAHL006102 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI FERRÉ-D'AMARÉ, ADRIAN · 2011 to 2025
$14.6M
RNA: Tools for Cell BiologyZIAHL006188 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI FERRÉ-D'AMARÉ, ADRIAN · 2014 to 2025
$9.5M
Intramural NIH HHS Z99 HL999999Intramural NIH HHS ZIA HL006102
6 · The paper itself

Abstract

Genetically encoded fluorescent protein tags have revolutionized proteome studies, whereas the lack of intrinsically fluorescent RNAs has hindered transcriptome exploration. Among several RNA-fluorophore complexes that potentially address this problem, RNA Mango has an exceptionally high affinity for its thiazole orange (TO)-derived fluorophore, TO1-Biotin (K

Indexed as

BenzothiazolesBinding SitesFluorescent DyesLigandsQuinolinesRNABenzothiazolesFluorescent DyesLigandsQuinolinesRNAthiazole orange

Identifiers

PMID28553947
PMCPMC5550021
OpenAlexW2617151789

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.