ArticleLaboratory investigation; a journal of technical methods and pathology2017
Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections.
Article in Laboratory investigation; a journal of technical methods and pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Immunocytochemical staining for lymphatic and blood vessels with frozen sections: using LYVE-1 for lymphatic vessel and von Willebrand factor for blood vessel marker.European journal of histochemistry : EJH · 2026Article
- AI-augmented intraoperative decision-making workflows in diffuse midline glioma biopsy using cryosection pathology.Nature communications · 2025Article
- Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency.Engineering in life sciences · 2024Article
- Article
- Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study.Virchows Archiv : an international journal of pathology · 2019Article
- A microfluidic platform towards automated multiplexed in situ sequencing.Scientific reports · 2019Article
- Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections.Microsystems & nanoengineering · 2019Article
- Combining fluorescence-based image segmentation and automated microfluidics for ultrafast cell-by-cell assessment of biomarkers for HER2-type breast carcinoma.Journal of biomedical optics · 2018Article
- Microfluidic on-chip immunohistochemistry directly from a paraffin-embedded section.Biomicrofluidics · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Frozen sections (FS) of tumor samples represent a cornerstone of pathological intraoperative consultation and have an important role in the microscopic analysis of specimens during surgery. So far, immunohistochemical (IHC) stainings on FS have been demonstrated for a few markers using manual methods. Microfluidic technologies have proven to bring substantial improvement in many fields of diagnostics, though only a few microfluidic devices have been designed to improve the performance of IHC assays. In this work, we show optimization of a complete pan-cytokeratin chromogenic immunostaining protocol on FS using a microfluidic tissue processor into a protocol taking <12 min. Our results showed specificity and low levels of background. The dimensions of the microfluidic prototype device are compatible with the space constraints of an intraoperative pathology laboratory. We therefore anticipate that the adoption of microfluidic technologies in the field of surgical pathology can significantly improve the way FSs influence surgical procedures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.