ArticleCurrent biotechnology2015
Characterization of an Eukaryotic PL-7 Alginate Lyase in the Marine Red Alga
Article in Current biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 34 citations in OpenAlex.
- Analysis of unsaturated alginate oligosaccharides using high-performance anion exchange chromatography coupled with mass spectrometry.Analytical and bioanalytical chemistry · 2024Article
- Development of Chromatin Immunoprecipitation for the Analysis of Histone Modifications in Red Macroalga Neopyropia yezoensis (Rhodophyta).Molecular biotechnology · 2023Article
- Characterization of a Novel Alginate Lyase with Two Alginate Lyase Domains from the Marine BacteriumMarine drugs · 2022Article
- Article
- Determination of oligosaccharide product distributions of PL7 alginate lyases by their structural elements.Communications biology · 2022Article
- Alginate Lyases from Marine Bacteria: An Enzyme Ocean for Sustainable Future.Molecules (Basel, Switzerland) · 2022Review
- Phospholipase D activation is required for 1-aminocyclopropane 1-carboxylic acid signaling during sexual reproduction in the marine red alga Neopyropia yezoensis (Rhodophyta).BMC plant biology · 2022Article
- Evolving strategies for marine enzyme engineering: recent advances on the molecular modification of alginate lyase.Marine life science & technology · 2022Review
- Comparative Gene Expression and Physiological Analyses Reveal Molecular Mechanisms in Wound-Induced Spore Formation in the Edible Seaweed Nori.Frontiers in plant science · 2022Article
- Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases.Marine drugs · 2021Review
- Specificities and Synergistic Actions of Novel PL8 and PL7 Alginate Lyases from the Marine FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Recent progress on engineering microbial alginate lyases towards their versatile role in biotechnological applications.Folia microbiologica · 2020Review
- Structural and molecular basis for the substrate positioning mechanism of a new PL7 subfamily alginate lyase from the arctic.The Journal of biological chemistry · 2020Article
- Purification and Characterization of a Novel Endolytic Alginate Lyase fromMarine drugs · 2020Article
- Proteomic enzyme analysis of the marine fungus Paradendryphiella salina reveals alginate lyase as a minimal adaptation strategy for brown algae degradation.Scientific reports · 2019Article
- Rational Design of Alginate Lyase fromMarine drugs · 2019Article
- A unique life cycle transition in the red seaweedCommunications biology · 2019Article
- Characterization of a Novel PolyM-Preferred Alginate Lyase from MarineMarine drugs · 2018Article
- Biochemical Characterization and Degradation Pattern of a Novel Endo-Type Bifunctional Alginate Lyase AlyA from Marine BacteriumMarine drugs · 2018Article
- Purification and Characterization of a Novel Alginate Lyase from the Marine Bacterium Bacillus sp. Alg07.Marine drugs · 2018Article
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Authors and funding
6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundAlginate lyases belonging to polysaccharide lyase family-7 (PL-7) are the most well studied on their structures and functions among whole alginate lyases. However, all characterized PL-7 alginate lyases are from prokaryotic bacteria cells. Here we report the first identification of eukaryotic PL-7 alginate lyase from marine red alga Pyropia yezoensis.
methodsThe cDNA encoding an alginate lyase PyAly was cloned and was used for the construction of recombinant PyAly (rPyAly) expression system in Escherichia coli. Purified rPyAly was assayed to identify its enzymatic properties. Its expression pattern in P. yessoensis was also investigated.
resultsPyAly is likely a secreted protein consisting of an N-terminal signal peptide of 25 residues and a catalytic domain of 216 residues. The amino-acid sequence of the catalytic domain showed 19-29% identities to those of bacterial characterized alginate lyases classified into family PL-7. Recombinant PyAly protein, rPyAly, which was produced with E. coli BL21(DE3) by cold-inducible expression system, drastically decreased the viscosity of alginate solution in the early stage of reaction. The most preferable substrate for rPyAly was the poly(M) of alginate with an optimal temperature and pH at 35
conclusionThis study demonstrates that PyAly in marine red alga P. yezoensis is a novel PL-7 alginate lyase with an endolytic manner. PyAly is a gametophyte-specifically expressed protein and its structural gene is composed of four exons and three introns. Thus, PyAly is the first enzymatically characterized eukaryotic PL-7 alginate lyase.
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