ArticleChemical science2017
Light-up probe based on AIEgens: dual signal turn-on for caspase cascade activation monitoring.
Article in Chemical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 101 citations in OpenAlex.
- A Caspase-3-Activatable Near-Infrared AIEgen for Tumor Apoptosis Imaging In Vivo.Chemical & biomedical imaging · 2026Article
- Lipid Nanovesicles in Cancer Treatment: Improving Targeting and Stability of Antisense Oligonucleotides.Drug design, development and therapy · 2025Review
- Dual-locked fluorescent probes for precise diagnosis and targeted treatment of tumors.Heliyon · 2024Review
- Stimuli-responsive linkers and their application in molecular imaging.Exploration (Beijing, China) · 2024Review
- Selective chemical reagents to investigate the role of caspase 6 in apoptosis in acute leukemia T cells.Chemical science · 2023Article
- Review
- Activated molecular probes for enzyme recognition and detection.Theranostics · 2022Review
- Dual-locked spectroscopic probes for sensing and therapy.Nature reviews. Chemistry · 2021Review
- Review
- Protease-responsive mass barcoded nanotranslators for simultaneously quantifying the intracellular activity of cascaded caspases in apoptosis pathways.Chemical science · 2020Article
- A sequence-activated AND logic dual-channel fluorescent probe for tracking programmable drug release.Chemical science · 2018Article
- Fluorometric probing of the lipase level as acute pancreatitis biomarkers based on interfacially controlled aggregation-induced emission (AIE).Chemical science · 2017Article
- Visualization of drug delivery processes using AIEgens.Chemical science · 2017Article
- Inhibition of Tumor Growth of Human Hepatocellular Carcinoma HepG2 Cells in a Nude Mouse Xenograft Model by the Total Flavonoids fromEvidence-based complementary and alternative medicine : eCAM · 2017Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct monitoring of multiple enzyme activities in a given biological process is extremely important for disease diagnosis. Herein, we report a single fluorescent probe that targets two caspase activities in living cells. The probe consists of three parts that includes two AIE fluorogens with distinctive green and red emission colors excitable at a single wavelength, and a hydrophilic peptide as the substrate of the apoptosis initiator caspase-8 and the effector caspase-3. The probe is non-fluorescent in aqueous media. The green and red fluorescence can be sequentially turned on when the peptide substrate is cleaved by the cascade activation of caspase-8 and caspase-3 in early apoptotic HeLa cells induced by hydrogen peroxide. This sequential fluorescence turn-on allows real-time monitoring of the caspase cascade activation during the apoptotic process, which was further explored for evaluating the therapeutic efficiency of anticancer drugs. The probe design strategy developed in this study also proved to be general, which opens a new avenue for real-time, multiplexed imaging of cellular enzyme activity in a biological process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.