Evidence map›Paper›PMID 28552605›Full record

ArticleStem cell reports2017

Defined and Scalable Differentiation of Human Oligodendrocyte Precursors from Pluripotent Stem Cells in a 3D Culture System.

Gonçalo M C Rodrigues, Thomas Gaj, Maroof M Adil, Joyce Wahba, Antara T Rao, Franziska K Lorbeer, Rishi U Kulkarni, Maria Margarida Diogo, Joaquim M S Cabral, Evan W Miller and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 63 citations in OpenAlex.

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  18. iPSC modeling of rare pediatric disorders.Journal of neuroscience methods · 2020
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Gonçalo M C RodriguesDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720-1762, USA; Department of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal.
Thomas GajDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720-1762, USA.
Maroof M AdilDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720-1462, USA.
Joyce WahbaDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720-1462, USA.
Antara T RaoDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720-1462, USA.
Franziska K LorbeerDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3370, USA.
Rishi U KulkarniDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720-1462, USA.
Maria Margarida DiogoDepartment of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal.
Joaquim M S CabralDepartment of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal.
Evan W MillerDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720-1462, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3370, USA; The Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720-3370, USA.
Dirk HockemeyerDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3370, USA.
David V SchafferDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720-1762, USA; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720-1462, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3370, USA; The Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720-3370, USA. Electronic address: schaffer@berkeley.edu.
University of California, Berkeley · USInstitute for Biotechnology and Bioengineering · PTUniversity of Lisbon · PT

Funding

Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
Telomerase function in human tumor and stem cell biologyR01CA196884 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI HOCKEMEYER, DIRK · 2015 to 2019
$1.7M
Molecular and optogenetic tools for studying voltage in the brainR00NS078561 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI MILLER, EVAN WALKER · 2013 to 2015
$713k
Therapeutic genome editing for amyotrophic lateral sclerosisF32GM113446 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI GAJ, THOMAS · 2015 to 2017
$125k
NCI NIH HHS R01 CA196884NIGMS NIH HHS F32 GM113446NIGMS NIH HHS T32 GM066698NINDS NIH HHS R00 NS078561
6 · The paper itself

Abstract

Oligodendrocyte precursor cells (OPCs) offer considerable potential for the treatment of demyelinating diseases and injuries of the CNS. However, generating large quantities of high-quality OPCs remains a substantial challenge that impedes their therapeutic application. Here, we show that OPCs can be generated from human pluripotent stem cells (hPSCs) in a three-dimensional (3D), scalable, and fully defined thermoresponsive biomaterial system. We used CRISPR/Cas9 to create a NKX2.2-EGFP human embryonic stem cell reporter line that enabled fine-tuning of early OPC specification and identification of conditions that markedly increased the number of OLIG2

Indexed as

Cell DifferentiationAnimalsBiocompatible MaterialsBrainCell Culture TechniquesCell LineCellular ReprogrammingCRISPR-Cas SystemsGenes, ReporterHomeobox Protein Nkx-2.2Homeodomain ProteinsHumansMiceMice, Inbred NODMice, SCIDNuclear ProteinsBiocompatible MaterialsHomeobox Protein Nkx-2.2Homeodomain Proteinsnkx2.2b protein, zebrafishNKX2-2 protein, humanNkx2-2 protein, mouseNuclear ProteinsOligodendrocyte Transcription Factor 2Transcription FactorsZebrafish ProteinsCRISPR/Cas9 knockinhuman pluripotent stem cellsNKX2.2-EGFP reporter cell lineoligodendrocyte precursor cellsscalable and defined oligodendrocyte differentiationthree-dimensional culture systems

Identifiers

PMID28552605
PMCPMC5470111
OpenAlexW2617547981

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.