ArticleCurrent biology : CB2017
The THO Complex Non-Cell-Autonomously Represses Female Germline Specification through the TAS3-ARF3 Module.
Article in Current biology : CB, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 100 citations in OpenAlex.
- Review
- The Arabidopsis tex1-4 mutant exhibits altered mRNA export and hormonal crosstalk in plant immunity against Pseudomonas syringae.Plant cell reports · 2026Article
- VPS13 has an important role in female germline development in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- MicroRNA networks in rice seeds: unveiling key regulators of development, germination, and rice resilience under climate changes.Journal of experimental botany · 2026Review
- The tasiR-ARF pathway in plants: origin, functions, and interplay of miR-390, tasiRNAs and ARF3.Plant biology (Stuttgart, Germany) · 2026Review
- A Noncanonical Auxin-Sensing Mechanism Uncovered by Screening the Auxin Response Factor 3 Interacting Proteins in Tomato.International journal of molecular sciences · 2026Article
- SPOROCYTELESS/NOZZLE cooperates with MADS-domain transcription factors to regulate an auxin-dependent network controlling Megaspore-Mother-Cell differentiation.Nature communications · 2025Article
- Recovery of RNA-dependent RNA polymerase 6 gene-knockout phenotypes in Nicotiana benthamiana via in vivo generation of inverted repeat construct of the trans-acting short interference RNA3 sequence.The Plant journal : for cell and molecular biology · 2025Article
- Sexual reproduction in land plants: an evolutionary perspective.Plant reproduction · 2025Review
- Advances in Small RNA Regulation of Female Gametophyte Development in Flowering Plants.Plants (Basel, Switzerland) · 2025Review
- The DNA methylation-demethylation balance prevents development of multiple megaspore mother cells in Arabidopsis.The Plant cell · 2025Article
- Epigenetic gene regulation in plants and its potential applications in crop improvement.Nature reviews. Molecular cell biology · 2025Review
- Whole Mount Staining and Immunolocalization Procedures for Developing Ovules of Vigna unguiculata.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Ribosomes binding toFrontiers in plant science · 2025Article
- Auxin response factor 10 insensitive to miR160regulation induces apospory-like phenotypes iniScience · 2024Article
- Revisiting the female germline cell development.Frontiers in plant science · 2024Review
- Small RNA-mediated DNA methylation during plant reproduction.The Plant cell · 2023Review
- Beyond transcription: compelling open questions in plant RNA biology.The Plant cell · 2023Article
- Apomixis: oh, what a tangled web we have!Planta · 2023Review
- A model worker: Multifaceted modulation of AUXIN RESPONSE FACTOR3 orchestrates plant reproductive phases.Frontiers in plant science · 2023Review
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
In most sexually reproducing plants, a single somatic, sub-epidermal cell in an ovule is selected to differentiate into a megaspore mother cell, which is committed to giving rise to the female germline. However, it remains unclear how intercellular signaling among somatic cells results in only one cell in the sub-epidermal layer differentiating into the megaspore mother cell. Here we uncovered a role of the THO complex in restricting the megaspore mother cell fate to a single cell. Mutations in TEX1, HPR1, and THO6, components of the THO/TREX complex, led to the formation of multiple megaspore mother cells, which were able to initiate gametogenesis. We demonstrated that TEX1 repressed the megaspore mother cell fate by promoting the biogenesis of TAS3-derived trans-acting small interfering RNA (ta-siRNA), which represses ARF3 expression. The TEX1 protein was present in epidermal cells, but not in the germline, and, through TAS3-derived ta-siRNA, restricted ARF3 expression to the medio domain of ovule primordia. Expansion of ARF3 expression into lateral epidermal cells in a TAS3 ta-siRNA-insensitive mutant led to the formation of supernumerary megaspore mother cells, suggesting that TEX1- and TAS3-mediated restriction of ARF3 expression limits excessive megaspore mother cell formation non-cell-autonomously. Our findings reveal the role of a small-RNA pathway in the regulation of female germline specification in Arabidopsis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.