ArticleACS infectious diseases2017
Optimized Fluorescence Complementation Platform for Visualizing Salmonella Effector Proteins Reveals Distinctly Different Intracellular Niches in Different Cell Types.
Article in ACS infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Engineering a new tripartite split-ccGFP system from Corynactis californica for detecting protein-protein interactions.Scientific reports · 2025Article
- Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Engineering highly stable variants of Corynactis californica green fluorescent proteins.Protein science : a publication of the Protein Society · 2024Article
- Visualizing the invisible: novel approaches to visualizing bacterial proteins and host-pathogen interactions.Frontiers in bioengineering and biotechnology · 2024Review
- Speaking the host language: howMicrobiology (Reading, England) · 2023Review
- Recent Advancements in Tracking Bacterial Effector Protein Translocation.Microorganisms · 2022Review
- Engineering an efficient and bright split Corynactis californica green fluorescent protein.Scientific reports · 2021Article
- Genetic code expansion enables visualization ofeLife · 2021Article
- Bacterial injection machines: Evolutionary diverse but functionally convergent.Cellular microbiology · 2020Article
- Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology.International journal of molecular sciences · 2019Review
- Article
- A Multicolor Split-Fluorescent Protein Approach to Visualize Listeria Protein Secretion in Infection.Biophysical journal · 2018Article
- Tracking elusive cargo: Illuminating spatio-temporal Type 3 effector protein dynamics using reporters.Cellular microbiology · 2018Review
- Methods to Illuminate the Role ofFrontiers in cellular and infection microbiology · 2017Review
- Review
- What the SIF Is Happening-The Role of IntracellularFrontiers in cellular and infection microbiology · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The bacterial pathogen Salmonella uses sophisticated type III secretion systems (T3SS) to translocate and deliver bacterial effector proteins into host cells to establish infection. Monitoring these important virulence determinants in the context of live infections is a key step in defining the dynamic interface between the host and pathogen. Here, we provide a modular labeling platform based on fluorescence complementation with split-GFP that permits facile tagging of new Salmonella effector proteins. We demonstrate enhancement of split-GFP complementation signals by manipulating the promoter or by multimerizing the fluorescent tag and visualize three effector proteins, SseF, SseG, and SlrP, that have never before been visualized over time during infection of live cells. Using this platform, we developed a methodology for visualizing effector proteins in primary macrophage cells for the first time and reveal distinct differences in the effector-defined intracellular niche between primary macrophage and commonly used HeLa and RAW cell lines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.