ArticleCytotechnology2018
Development of an 8-color antibody panel for functional phenotyping of human CD8+ cytotoxic T cells from peripheral blood mononuclear cells.
Article in Cytotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Article
- Expansion, persistence, and efficacy of donor memory-like NK cells infused for posttransplant relapse.The Journal of clinical investigation · 2022Article
- Combining CTLA-4 and angiopoietin-2 blockade in patients with advanced melanoma: a phase I trial.Journal for immunotherapy of cancer · 2021Article
- Immune modulating activity of the CHK1 inhibitor prexasertib and anti-PD-L1 antibody LY3300054 in patients with high-grade serous ovarian cancer and other solid tumors.Cancer immunology, immunotherapy : CII · 2021Article
- High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples.STAR protocols · 2020Article
- Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.Journal of biological methods · 2020Article
- Anti-PD-1 Immunotherapy-Induced Flare of a Known Underlying Relapsing Vasculitis Mimicking Recurrent Cancer.The oncologist · 2019Article
- Detection of clinically relevant immune checkpoint markers by multicolor flow cytometry.Journal of biological methods · 2019Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of CD8 positive cells in peripheral blood has become an essential part of research in the field of cancer immunotherapies, vaccine development, inflammation, autoimmune disease, etc. In this study, an 8-color flow cytometry panel, containing lineage and functional markers, was developed for the identification of CD8+ cytotoxic T cells in previously cryopreserved peripheral blood mononuclear cells from healthy human donors. By studying functional markers in naïve and CD3/CD28 activated T cells we demonstrate that the panel is capable of detecting protein markers corresponding to different T cell activation statuses. Data generated by flow cytometry were corroborated by different antibody based assay technologies to detect soluble cytokines. Our findings suggest that there is an inter donor variability in both baseline and activation responses. We have also successfully developed an antibody panel for flow cytometry that could be used to study cytotoxic function of CD8 T cells in clinical immunology research areas.
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