ArticleThe Journal of infection2017
An evaluation of purified Salmonella Typhi protein antigens for the serological diagnosis of acute typhoid fever.
Article in The Journal of infection, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Protein-centric mechanisms of typhoidalFrontiers in cellular and infection microbiology · 2026Review
- Leveraging paired serology to estimate the incidence of typhoidal Salmonella infection in the STRATAA study.PLoS neglected tropical diseases · 2025Article
- The Identification of Enteric Fever-Specific Antigens for Population-Based Serosurveillance.The Journal of infectious diseases · 2024Article
- Typhoid fever.Nature reviews. Disease primers · 2023Review
- Bacterial shedding and serologic responses following an outbreak of Salmonella Typhi in an endemic cohort.BMC infectious diseases · 2023Article
- Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia.BMC infectious diseases · 2022Article
- An age-stratified serosurvey against purified Salmonella enterica serovar Typhi antigens in the Lao People´s Democratic Republic.PLoS neglected tropical diseases · 2021Article
- Performance of Immunodiagnostic Tests for Typhoid Fever: A Systematic Review and Meta-Analysis.Pathogens (Basel, Switzerland) · 2021Review
- Enteric Fever Diagnosis: Current Challenges and Future Directions.Pathogens (Basel, Switzerland) · 2021Review
- Salmonella Typhoid Toxin PltB Subunit and Its Non-typhoidal Salmonella Ortholog Confer Differential Host Adaptation and Virulence.Cell host & microbe · 2020Article
- The role of 9-O-acetylated glycan receptor moieties in the typhoid toxin binding and intoxication.PLoS pathogens · 2020Article
- Ascertaining the burden of invasive Salmonella disease in hospitalised febrile children aged under four years in Blantyre, Malawi.PLoS neglected tropical diseases · 2019Article
- The Design and Analysis of Seroefficacy Studies for Typhoid Conjugate Vaccines.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Serodiagnostic evaluation of recombinant CdtB of S. Typhi as a potential candidate for acute typhoid.Immunologic research · 2018Article
- Why Is Eradicating Typhoid Fever So Challenging: Implications for Vaccine and Therapeutic Design.Vaccines · 2018Review
- Typhoid Fever Diagnosis in Endemic Countries: A Clog in the Wheel of Progress?Medicina (Kaunas, Lithuania) · 2018Review
- In vivo tropism of Salmonella Typhi toxin to cells expressing a multiantennal glycan receptor.Nature microbiology · 2018Article
Corrections and comments
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Authors and funding
29 authors.
Funding
Abstract
objectivesThe diagnosis of typhoid fever is a challenge. Aiming to develop a typhoid diagnostic we measured antibody responses against Salmonella Typhi (S. Typhi) protein antigens and the Vi polysaccharide in a cohort of Bangladeshi febrile patients.
methodsIgM against 12 purified antigens and the Vi polysaccharide was measured by ELISA in plasma from patients with confirmed typhoid fever (n = 32), other confirmed infections (n = 17), and healthy controls (n = 40). ELISAs with the most specific antigens were performed on plasma from 243 patients with undiagnosed febrile disease.
resultsIgM against the S. Typhi protein antigens correlated with each other (rho > 0.8), but not against Vi (rho < 0.6). Typhoid patients exhibited higher IgM against 11/12 protein antigens and Vi than healthy controls and those with other infections. Vi, PilL, and CdtB exhibited the greatest sensitivity and specificity. Specificity and sensitivity was improved when Vi was combined with a protein antigen, generating sensitivities and specificities of 0.80 and >0.85, respectively. Applying a dynamic cut-off to patients with undiagnosed febrile disease suggested that 34-58% had an IgM response indicative of typhoid.
conclusionsWe evaluated the diagnostic potential of several S. Typhi antigens; our assays give good sensitivity and specificity, but require further assessment in differing patient populations.
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