ArticleThe Journal of surgical research2017
pH modulation ameliorates the red blood cell storage lesion in a murine model of transfusion.
Article in The Journal of surgical research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The Proteasome Safeguards Red Blood Cell Integrity During Storage and After Transfusion.Antioxidants (Basel, Switzerland) · 2026Article
- Cellular and Molecular Mechanisms of Hemorrhagic Shock: Biological Rationale for Individualized Fluid Resuscitation Strategies and Multimodal Monitoring.Biomedicines · 2026Review
- Feasibility and effectiveness of the novel HemoClear microfiltration cell-salvage system in cesarean sections in South Africa.International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics · 2026Article
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- Toward Translational Impact of Low-Glucose Strategies on Red Blood Cell Storage Optimization.ACS pharmacology & translational science · 2024Article
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- Evaluation of selected hematological, biochemical and oxidative stress parameters in stored canine CPDA-1 whole blood.BMC veterinary research · 2022Article
- Multiparametric characterization of red blood cell physiology after hypotonic dialysis based drug encapsulation process.Acta pharmaceutica Sinica. B · 2022Article
- Improving packed red blood cell storage with a high-viscosity buffered storage solution.Surgery · 2022Article
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- Magnetophoretic and spectral characterization of oxyhemoglobin and deoxyhemoglobin: Chemical versus enzymatic processes.PloS one · 2021Article
- The therapeutic importance of acid-base balance.Biochemical pharmacology · 2021Review
- Attenuation of Red Blood Cell Storage Lesions with Vitamin C.Antioxidants (Basel, Switzerland) · 2017Article
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Authors and funding
5 authors.
Funding
Abstract
backgroundProlonged storage of packed red blood cells (pRBCs) induces a series of harmful biochemical and metabolic changes known as the RBC storage lesion. RBCs are currently stored in an acidic storage solution, but the effect of pH on the RBC storage lesion is unknown. We investigated the effect of modulation of storage pH on the RBC storage lesion and on erythrocyte survival after transfusion.
methodsMurine pRBCs were stored in Additive Solution-3 (AS3) under standard conditions (pH, 5.8), acidic AS3 (pH, 4.5), or alkalinized AS3 (pH, 8.5). pRBC units were analyzed at the end of the storage period. Several components of the storage lesion were measured, including cell-free hemoglobin, microparticle production, phosphatidylserine externalization, lactate accumulation, and byproducts of lipid peroxidation. Carboxyfluorescein-labeled erythrocytes were transfused into healthy mice to determine cell survival.
resultsCompared with pRBCs stored in standard AS3, those stored in alkaline solution exhibited decreased hemolysis, phosphatidylserine externalization, microparticle production, and lipid peroxidation. Lactate levels were greater after storage in alkaline conditions, suggesting that these pRBCs remained more metabolically viable. Storage in acidic AS3 accelerated erythrocyte deterioration. Compared with standard AS3 storage, circulating half-life of cells was increased by alkaline storage but decreased in acidic conditions.
conclusionsStorage pH significantly affects the quality of stored RBCs and cell survival after transfusion. Current erythrocyte storage solutions may benefit from refinements in pH levels.
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