ArticleScientific reports2017
The pPSU Plasmids for Generating DNA Molecular Weight Markers.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- A flexible, cost- and time-saving method for in-house laboratory production of DNA markers.Biotechnology letters · 2025Article
- pHAPE: a plasmid for production of DNA size marker ladders for gel electrophoresis.Biology methods & protocols · 2023Article
- Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories.AMB Express · 2022Article
- The Plasmodium NOT1-G paralogue is an essential regulator of sexual stage maturation and parasite transmission.PLoS biology · 2021Article
- The Penn State Protein Ladder system for inexpensive protein molecular weight markers.Scientific reports · 2021Article
- Ribozyme-mediated, multiplex CRISPR gene editing and CRISPR interference (CRISPRi) in rodent-infectiousThe Journal of biological chemistry · 2019Article
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Authors and funding
4 authors.
Funding
Abstract
Visualizing nucleic acids by gel electrophoresis is one of the most common techniques in molecular biology, and reference molecular weight markers or ladders are commonly used for size estimation. We have created the pPSU1 & pPSU2 pair of molecular weight marker plasmids which produce both 100 bp and 1 kb DNA ladders when digested with two common restriction enzymes. The 100 bp ladder fragments have been optimized to migrate appropriately on both agarose and native polyacrylamide, unlike many currently available DNA ladders. Sufficient plasmid DNA can be isolated from 100 ml E. coli cultures for the two plasmids to produce 100 bp or 1 kb ladders for 1000 gels. As such, the pPSU1 and pPSU2 plasmids provide reference fragments from 50 to 10000 bp at a fraction of the cost of commercial DNA ladders. The pPSU1 and pPSU2 plasmids are available without licensing restrictions to nonprofit academic users, affording freely available high-quality, low-cost molecular weight standards for molecular biology applications.
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Registered trials
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