ArticleScientific reports2017
Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Identification of Antibody-Cognate Antigens via a High-Throughput, Affinity Chromatography-Based, Shotgun Immunoproteomics Pipeline.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Ultrastructural assessment of human periodontal ligament fibroblast interaction with bovine pericardium membranes: AnHistology and histopathology · 2025Article
- The efficacy of an embryonic stem cell-based vaccine for lung cancer prevention depends on the undifferentiated state of the stem cells.Scientific reports · 2024Article
- Current Challenges and Future Promise for Use of Extracellular Matrix Scaffold to Achieve the Whole Organ Tissue Engineering Moonshot.Stem cells translational medicine · 2023Review
- Optimization of a high-throughput shotgun immunoproteomics pipeline for antigen identification.Journal of proteomics · 2023Article
- Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine.Biomaterials research · 2023Review
- Preliminary study of improving immune tolerance in vivo of bioprosthetic heart valves through a novel antigenic removal method.Frontiers in bioengineering and biotechnology · 2023Article
- Long-Term Stability and Biocompatibility of Pericardial Bioprosthetic Heart Valves.Frontiers in cardiovascular medicine · 2021Review
- Immunoproteomic Identification of Noncarbohydrate Antigens Eliciting Graft-Specific Adaptive Immune Responses in Patients with Bovine Pericardial Bioprosthetic Heart Valves.Proteomics. Clinical applications · 2019Article
- Recipients with blood group A associated with longer survival rates in cardiac valvular bioprostheses.EBioMedicine · 2019Article
- Graft-specific immune tolerance is determined by residual antigenicity of xenogeneic extracellular matrix scaffolds.Acta biomaterialia · 2018Article
- Cardiac Non-Human Leukocyte Antigen Identification: Techniques and Troubles.Frontiers in immunology · 2017Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Despite bovine pericardium (BP) being the primary biomaterial used in heart valve bioprostheses, recipient graft-specific immune responses remain a significant cause of graft failure. Consequently, tissue antigenicity remains the principal barrier for expanding use of such biomaterials in clinical practice. We hypothesize that our understanding of BP antigenicity can be improved by application of a combined affinity chromatography shotgun immunoproteomic approach to identify antigens that have previously been overlooked. Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) analysis of affinity chromatography purified antigens resulted in identification of 133 antigens. Most importantly, antigens were identified from all subcellular locations, including 18 integral membrane protein antigens. Critically, isoforms of several protein families were found to be antigenic suggesting the possibility that shared epitope domains may exist. Furthermore, proteins associated with immune, coagulation, and inflammatory pathways were over-represented, suggesting that these biological processes play a key role in antigenicity. This study brings to light important determinants of antigenicity in a clinically relevant xenogeneic biomaterial (i.e. BP) and further validates a rapid, high-throughput method for immunoproteomic antigen identification.
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Registered trials
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