ArticleBiochimica et biophysica acta. Biomembranes2017
Topographic analysis by atomic force microscopy of proteoliposomes matrix vesicle mimetics harboring TNAP and AnxA5.
Article in Biochimica et biophysica acta. Biomembranes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Development and in vitro evaluation of liposomes and immunoliposomes containing 5-fluorouracil and R-phycoerythrin as a potential phototheranostic system for colorectal cancer.Beilstein journal of nanotechnology · 2026Article
- Automated morphological classification and quantification of cerebrospinal fluid extracellular vesiclesNanoscale advances · 2025Article
- Annexin A5 stabilizes matrix vesicle-biomimetic lipid membranes: unravelling a new role of annexins in calcification.European biophysics journal : EBJ · 2023Article
- Shedding Light on the Role of Na,K-ATPase as a Phosphatase during Matrix-Vesicle-Mediated Mineralization.International journal of molecular sciences · 2022Article
- Efficient Catalytic Reduction of Selected Toxic Dyes by Green Biosynthesized Silver Nanoparticles Using Aqueous Leaf Extract ofNanomaterials (Basel, Switzerland) · 2022Article
- Tools shaping drug discovery and development.Biophysics reviews · 2022Review
- Mineralization Profile of Annexin A6-Harbouring Proteoliposomes: Shedding Light on the Role of Annexin A6 on Matrix Vesicle-Mediated Mineralization.International journal of molecular sciences · 2022Article
- Article
- Fluorescence evidence of annexin A6 translocation across membrane in model matrix vesicles during apatite formation.Journal of extracellular biology · 2022Article
- Matrix vesicles from dental follicle cells improve alveolar bone regeneration via activation of the PLC/PKC/MAPK pathway.Stem cell research & therapy · 2022Article
- Electrically Tunable Lens (ETL)-Based Variable Focus Imaging System for Parametric Surface Texture Analysis of Materials.Micromachines · 2021Article
- Langmuir monolayers and proteoliposomes as models of matrix vesicles involved in biomineralization.Biophysical reviews · 2021Article
- Research and application of hydrostatic high pressure in tumor vaccines (Review).Oncology reports · 2021Review
- Article
- Phosphatidylserine controls calcium phosphate nucleation and growth on lipid monolayers: A physicochemical understanding of matrix vesicle-driven biomineralization.Journal of structural biology · 2020Article
- Lipid composition modulates ATP hydrolysis and calcium phosphate mineral propagation by TNAP-harboring proteoliposomes.Archives of biochemistry and biophysics · 2020Article
- Matrix vesicle biomimetics harboring Annexin A5 and alkaline phosphatase bind to the native collagen matrix produced by mineralizing vascular smooth muscle cells.Biochimica et biophysica acta. General subjects · 2020Article
- Localization of Annexin A6 in Matrix Vesicles During Physiological Mineralization.International journal of molecular sciences · 2020Article
- Cholesterol Regulates the Incorporation and Catalytic Activity of Tissue-Nonspecific Alkaline Phosphatase in DPPC Monolayers.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Quantitative atomic force microscopy provides new insight into matrix vesicle mineralization.Archives of biochemistry and biophysics · 2019Article
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8 authors.
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Abstract
Atomic force microscopy (AFM) is one of the most commonly used scanning probe microscopy techniques for nanoscale imaging and characterization of lipid-based particles. However, obtaining images of such particles using AFM is still a challenge. The present study extends the capabilities of AFM to the characterization of proteoliposomes, a special class of liposomes composed of lipids and proteins, mimicking matrix vesicles (MVs) involved in the biomineralization process. To this end, proteoliposomes were synthesized, composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phospho-l-serine (DPPS), with inserted tissue-nonspecific alkaline phosphatase (TNAP) and/or annexin V (AnxA5), both characteristic proteins of osteoblast-derived MVs. We then aimed to study how TNAP and AnxA5 insertion affects the proteoliposomes' membrane properties and, in turn, interactions with type II collagen, thus mimicking early MV activity during biomineralization. AFM images of these proteoliposomes, acquired in dynamic mode, revealed the presence of surface protrusions with distinct viscoelasticity, thus suggesting that the presence of the proteins induced local changes in membrane fluidity. Surface protrusions were measurable in TNAP-proteoliposomes but barely detectable in AnxA5-proteoliposomes. More complex surface structures were observed for proteoliposomes harboring both TNAP and AnxA5 concomitantly, resulting in a lower affinity for type II collagen fibers compared to proteoliposomes harboring AnxA5 alone. The present study achieved the topographic analysis of lipid vesicles by direct visualization of structural changes, resulting from protein incorporation, without the need for fluorescent probes.
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