ArticleAMB Express2017
Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine.
Article in AMB Express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 33 citations in OpenAlex.
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- Physiological and Biochemical Analysis Revealing the Key Factors Influencing 2-Phenylethanol and Benzyl Alcohol Production in Crabapple Flowers.Plants (Basel, Switzerland) · 2024Article
- Investigating the biosynthesis and roles of the auxin phenylacetic acid duringFrontiers in plant science · 2024Article
- The Combined Effect of Lactic Acid Bacteria andMolecules (Basel, Switzerland) · 2023Article
- Article
- Construction of recombinant Escherichia coli for production of L-phenylalanine-derived compounds.World journal of microbiology & biotechnology · 2021Review
- The Use of Sour and Sweet Whey in Producing Compositions with Pleasant Aromas Using the MoldJournal of agricultural and food chemistry · 2020Article
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phenylacetic acid (PAA) is a fine chemical with a high industrial demand for its widespread uses. Whereas, microorganic synthesis of PAA is impeded by the formation of by-product phenethyl alcohol due to quick, endogenous, and superfluous conversion of aldehydes to their corresponding alcohols, which resulted in less conversation of PAA from aldehydes. In this study, an Escherichia coli K-12 MG1655 strain with reduced aromatic aldehyde reduction (RARE) that does duty for a platform for aromatic aldehyde biosynthesis was used to prompt more PAA biosynthesis. We establish a microbial biosynthetic pathway for PAA production from the simple substrate phenylalanine in E. coli with heterologous coexpression of aminotransferase (ARO8), keto acid decarboxylase (KDC) and aldehyde dehydrogenase H (AldH) gene. It was found that PAA transformation yield was up to ~94% from phenylalanine in E. coli and there was no by-product phenethyl alcohol was detected. Our results reveal the high efficiency of the RARE strain for production of PAA and indicate the potential industrial applicability of this microbial platform for PAA biosynthesis.
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