ArticleNucleic acids research2017
Evidence for double-strand break mediated mitochondrial DNA replication in Saccharomyces cerevisiae.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Caloric restriction enhances inheritance of wild-type mitochondrial DNA in Saccharomyces cerevisiae.Scientific reports · 2025Article
- HOXC11-mediated regulation of mitochondrial function modulates chemoresistance in colorectal cancer.BMC cancer · 2024Article
- What we know so far and what we can expect next: A molecular investigation of plant parasitism.Genetics and molecular biology · 2024Article
- Two mitochondrial HMG-box proteins, Cim1 and Abf2, antagonistically regulate mtDNA copy number in Saccharomyces cerevisiae.Nucleic acids research · 2023Article
- Review
- Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi.Nature communications · 2020Article
- Safeguarding mitochondrial genomes in higher eukaryotes.Nature structural & molecular biology · 2020Review
- Discordant evolution of mitochondrial and nuclear yeast genomes at population level.BMC biology · 2020Article
- Review
- The intrinsic ability of double-stranded DNA to carry out D-loop and R-loop formation.Computational and structural biotechnology journal · 2020Review
- Prevention of mitochondrial genomic instability in yeast by the mitochondrial recombinase Mhr1.Scientific reports · 2019Article
- Saccharomyces cerevisiae Mhr1 can bind Xho I-induced mitochondrial DNA double-strand breaks in vivo.Mitochondrion · 2018Article
- Unveiling the mystery of mitochondrial DNA replication in yeasts.Mitochondrion · 2018Review
- Regulation of Small Mitochondrial DNA Replicative Advantage by Ribonucleotide Reductase inG3 (Bethesda, Md.) · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The mechanism of mitochondrial DNA (mtDNA) replication in Saccharomyces cerevisiae is controversial. Evidence exists for double-strand break (DSB) mediated recombination-dependent replication at mitochondrial replication origin ori5 in hypersuppressive ρ- cells. However, it is not clear if this replication mode operates in ρ+ cells. To understand this, we targeted bacterial Ku (bKu), a DSB binding protein, to the mitochondria of ρ+ cells with the hypothesis that bKu would bind persistently to mtDNA DSBs, thereby preventing mtDNA replication or repair. Here, we show that mitochondrial-targeted bKu binds to ori5 and that inducible expression of bKu triggers petite formation preferentially in daughter cells. bKu expression also induces mtDNA depletion that eventually results in the formation of ρ0 cells. This data supports the idea that yeast mtDNA replication is initiated by a DSB and bKu inhibits mtDNA replication by binding to a DSB at ori5, preventing mtDNA segregation to daughter cells. Interestingly, we find that mitochondrial-targeted bKu does not decrease mtDNA content in human MCF7 cells. This finding is in agreement with the fact that human mtDNA replication, typically, is not initiated by a DSB. Therefore, this study provides evidence that DSB-mediated replication is the predominant form of mtDNA replication in ρ+ yeast cells.
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Registered trials
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